Search results
5 hours ago · In cDNA synthesis, reverse transcription transforms RNA into complementary DNA (cDNA), providing a foundational material for subsequent library preparation and sequencing applications. The cDNA is prepared into a sequencing library, including adapter ligation and amplification steps. These steps vary depending on the sequencing platform used.
5 hours ago · DNA sequencing plays significant role to understand structure and functional differences of an organism. Gene of interest has to amplify prior than the sequencing with the help of technique known as polymerase chain reaction, which involved the steps like denaturation, annealing of primer, and extension of DNA strand using deoxy nucleotides.
5 hours ago · The study consisted of 4 major steps: 1. Preparation of isolated islets from human deceased donors (n = 85 batches); 2. Gene expression assays of 39 hypoxia-related genes in isolated islets; 3.
5 hours ago · Regulatory T cells (Tregs) are essential to maintain immune homeostasis by promoting self-tolerance. Reduced Treg numbers or functionality can lead to a loss of tolerance, increasing the risk of developing autoimmune diseases. An overwhelming variety of human Tregs has been described, based on either specific phenotype, tissue compartment, or pathological condition, yet the bulk of the ...
5 hours ago · This in turn favors the formation of the heterodimer with RXR, DNA binding and transcription of the target genes . The activation mechanism of PPARγ by endogenous lipids, including fatty acids or their metabolites such as 15-deoxy-delta-12,14-prostaglandin J2 (15d-PGJ2) and synthetic compounds such as thiazolidinediones, has been characterized ...
5 hours ago · The key transcription factor for vasculogenesis and angiopoiesis during placental development, VEGFA , was downregulated in TSCs NT compared to TSCs IVF. The CYP11A1 , HSD3B1 , and HSD11B2 mRNA expression levels were significantly lower in the TSCs NT than in the TSCs IVF (Fig. 3 E), suggesting that the TSCs NT group had a diminished steroid ...
