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  1. Mar 25, 2019 · Erika Toda, Arika Takebayashi, Takatoshi Kiba, Yuriko Osakabe, Hitoshi Sakakibara, Norio Kato & Takashi Okamoto. Department of Biological Sciences, Tokyo Metropolitan University,...

  2. en.wikipedia.org › wiki › Erika_TodaErika Toda - Wikipedia

    Erika Toda (戸田 恵梨香, Toda Erika, born August 17, 1988) is a Japanese actress. [1] [2] Career. Toda has starred in many Japanese television dramas, including Liar Game, [3] Code Blue, Ryusei no Kizuna, and Keizoku 2: SPEC. [4] . She has also had supporting roles in many other popular TV dramas, such as Boss, Nobuta wo Produce, Engine, and Gal Circle.

  3. Oct 27, 2020 · The POEMed-like rice ovules contained 10–20% sugar, with extremely high sucrose content (98%). Trancriptomic analysis revealed that the osgcs1 ovules had downregulation of starch biosynthetic ...

    • Yujiro Honma, Yujiro Honma, Prakash Babu Adhikari, Keiko Kuwata, Tomoko Kagenishi, Ken Yokawa, Michi...
    • 2020
  4. Dec 1, 1985 · An efficient DNA- and selectable-marker-free genome-editing system using zygotes in rice. The efficiency of direct gene transfer to plant protoplasts has been increased more than 1000 fold over ...

    • R. D. Shillito, M. W. Saul, J. Paszkowski, M. Müller, I. Potrykus
    • 1985
  5. PMID: 30911123. DOI: 10.1038/s41477-019-0386-z. Abstract. Technology involving the targeted mutagenesis of plants using programmable nucleases has been developing rapidly and has enormous potential in next-generation plant breeding.

    • Erika Toda, Narumi Koiso, Arika Takebayashi, Masako Ichikawa, Takatoshi Kiba, Keishi Osakabe, Yuriko...
    • 2019
  6. Jul 7, 2020 · The details of the vector construction are described in Toda et al. (2019). To construct the tRNA-gRNA units for single genome editing, amplify the target sequence by PCR using specific primers to obtain an artificial gene including tRNA and gRNA scaffold sequences.

  7. Jun 9, 2020 · Here, a protocol for CRISPR/Cas9-based genome editing using rice zygotes is described. The genome-editing system is constructed via polyethylene glycol/calcium-mediated transfection with CRISPR/Cas9 components in rice zygotes, which are produced by in vitro fertilization of isolated rice gametes.

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