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      • Trophozoites invade the small or large intestine to form cysts. Cysts are ellipsoidal, nonmotile and contain 2 to 4 nuclei with long- and short-curved rods. They are 9 to 13 μm by 7 to 9 μm in size and possess a thick refractile wall. Newly formed cysts pass in the feces.
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  2. Jun 29, 2018 · The microscopic identification of Giardia spp. in fecal samples is considered as the gold standard method for the diagnosis of giardiasis. This method is performed to detecting cysts and trophozoites.

    • Table 2

      Giardiasis has a global distribution and it is a common...

  3. Giardia duodenalis cysts in wet mounts under differential interference contrast (DIC) microscopy. Giardia duodenalis cysts are oval to ellipsoid and measure 8-19 µm (average 10-14 µm). Mature cysts have 4 nuclei, while immature cysts have two.

  4. Jun 12, 2023 · Demonstration by microscopy of Giardia trophozoites or cysts in stool specimens (sensitivity of 50 - 70%) ( J Clin Diagn Res 2014;8:DC04 ) Detection of Giardia antigen in stool (sensitivity of 95 - 100%) ( J Clin Diagn Res 2014;8:DC04 ) PCR assay for detecting Giardia in stool.

  5. Sep 7, 2020 · The variation in our results between microscopic examination using direct smears and the Ritchie technique is due to the ability of each technique for the detection of cyst and trophozoite. Direct smears detect both cyst and trophozoite stages while the Ritchie technique is applicable to the cyst stage only [ 10 ].

    • Amjad Alharbi, Fawzia H Toulah, Majed H Wakid, Esam Azhar, Suha Farraj, Ahmed A Mirza
    • 10.7759/cureus.10287
    • 2020
    • Cureus. 2020 Sep; 12(9): e10287.
  6. In bright-field microscopy, cysts appear ovoid to ellipsoid in shape and usually measure 11 to 14 μm (range: 8 to 19 μm). Immature and mature cysts have 2 and 4 nuclei, respectively. Intracytoplasmic fi brils are visible in cysts. Unstained cyst. Cyst stained with iodine. 2. Trichrome stain.

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  7. Nov 8, 2022 · Causes. Risk factors. Complications. Prevention. Overview. Giardia infection is an intestinal infection marked by stomach cramps, bloating, nausea and bouts of watery diarrhea. Giardia infection is caused by a microscopic parasite that is found worldwide, especially in areas with poor sanitation and unsafe water.

  8. Feb 16, 2024 · Updated: Feb 16, 2024. Author: Eric M Sieloff, MD; Chief Editor: Burt Cagir, MD, FACS more... Print. Approach Considerations. The traditional basis of diagnosis is identifying Giardia...

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