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  1. Nov 28, 2023 · The Basic Local Alignment Search Tool (BLAST) finds regions of local similarity between sequences. The program compares nucleotide or protein sequences to sequence databases and calculates the statistical significance of matches.

  2. Select the sequence database to run searches against. No BLAST database contains all the sequences at NCBI. BLAST databases are organized by informational content (nr, RefSeq, etc.) or by sequencing technique (WGS, EST, etc.). more...

  3. The Basic Local Alignment Search Tool (BLAST) finds regions of local similarity between protein or nucleotide sequences. The program compares nucleotide or protein sequences to sequence in a database and calculates the statistical significance of the matches.

  4. Mar 21, 2024 · BLASTn (Nucleotide BLAST): compares one or more nucleotide query sequences to a subject nucleotide sequence or a database of nucleotide sequences. This is useful when trying to determine the evolutionary relationships among different organisms (see Comparing two or more sequences below).

  5. Jun 23, 2008 · The blastn application searches a nucleotide query against a nucleotide database. To send the search to our servers and databases, add the –remote option: blastn –db nt –query nt.fsa –out results.out -remote . See more about this option in the section below, BLAST+ remote service.

  6. The Nucleotide database is a collection of sequences from several sources, including GenBank, RefSeq, TPA and PDB. Genome, gene and transcript sequence data provide the foundation for biomedical research and discovery.

  7. NCBI BLAST allows you to input a sequence from DNA, RNA or protein residues (amino acids) and find sequences that are identical or similar. To get to BLAST from the NCBI home page, click BLAST from the Popular Resources menu bar on the right of the page.

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