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- GUS, is short for glucuronidase, an enzyme in the bacterium E. coli. GUS is a good reporter for plants, as it does not occur naturally, and thus, has a low background. With some simple genetic techniques, one can attach the promoter of the gene you want to investigate to the GUS coding region.
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The GUS reporter system (GUS: β-glucuronidase) is a reporter gene system, particularly useful in plant molecular biology and microbiology. Several kinds of GUS reporter gene assay are available, depending on the substrate used. The term GUS staining refers to the most common of these, a histochemical technique.
Mar 13, 2017 · GUS, is short for glucuronidase, an enzyme in the bacterium E. coli. GUS is a good reporter for plants, as it does not occur naturally, and thus, has a low background. With some simple genetic techniques, one can attach the promoter of the gene you want to investigate to the GUS coding region.
GUS assay (using β-glucuronidase) is an excellent method for detecting a single cell by staining it blue without using any complicated equipment. The drawback is that the cells are killed in the process. It is particularly common in plant science. Blue-white screen is used in both bacteria and eukaryotic cells.
Plant Molecular Biology Manual. Jeong Hee Lee & Fritz Schöffl. 1001 Accesses. Abstract. β -Glucuronidase (GUS) is a very versatile reporter of gene expression that is frequently used in plant molecular biology.
- Jeong Hee Lee, Fritz Schöffl
- 1997
The enzyme ~-glucuronidase (GUS E.C.3.2.1.31) catalyses the hydrolysis of a wide variety of glucuronides. Its sub strates consist of D-glucuronic acid conju gated through a 13-0-glycosidic linkage ...
- Jefferson Ra
- 1989
The reporter gene was the uidA gene of Escherichia coli that encodes the enzyme β-glucuronidase (GUS). Since then, the uidA gene (commonly referred to as the gus gene) has become one of the most widely used reporter genes in plant molecular biology. (For a detailed description of the gus gene, see Chapter 1 .) Download protocol PDF. Keywords.
The transgenic Arabidopsis plants we use contain the GUS reporter gene under the control of the cor15a gene promoter, which responds to cold stress. Following induction by cold or other environmental signals, the gusA gene will respond by producing the enzyme beta-glucuronidase (GUS).
