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May 23, 2024 · Definition. Recombinant DNA technology involves using enzymes and various laboratory techniques to manipulate and isolate DNA segments of interest. This method can be used to combine (or splice) DNA from different species or to create genes with new functions. The resulting copies are often referred to as recombinant DNA.
May 23, 2024 · Definition. Mitochondrial DNA is the circular chromosome found inside the cellular organelles called mitochondria. Located in the cytoplasm, mitochondria are the site of the cell’s energy production and other metabolic functions. Offspring inherit mitochondria — and as a result mitochondrial DNA — from their mother.
May 23, 2024 · Definition. DNA sequencing refers to the general laboratory technique for determining the exact sequence of nucleotides, or bases, in a DNA molecule. The sequence of the bases (often referred to by the first letters of their chemical names: A, T, C, and G) encodes the biological information that cells use to develop and operate.
May 21, 2024 · An allele is a term coined to describe a specific copy of a gene. Genes, the DNA sequences controlling our traits, are usually found in two copies in eukaryotic genomes; each copy (allele) is inherited from one parent. Each allele occupies a specific region on the chromosome called a gene locus. The gene’s two alleles are located in the same ...
2 days ago · DNA extraction is often the initial step for molecular biology applications. When samples arrive in the lab, several techniques can be employed to isolate DNA for downstream applications such as polymerase chain reaction (PCR). In this article, we'll explore the different methods available and their equipment requirements, advantages and drawbacks. We'll also revise some outdated techniques ...
May 23, 2024 · Definition. cDNA (short for copy DNA; also called complementary DNA) is synthetic DNA that has been transcribed from a specific mRNA through a reaction using the enzyme reverse transcriptase. While DNA is composed of both coding and non-coding sequences, cDNA contains only coding sequences. Scientists often synthesize and use cDNA as a tool in ...
May 19, 2024 · The procedure for creating a DNA fingerprint consists of first obtaining a sample of cells, such as skin, hair, or blood cells, which contain DNA. The DNA is extracted from the cells and purified. In Jeffreys’s original approach, which was based on restriction fragment length polymorphism (RFLP) technology, the DNA was then cut at specific ...
