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- Inverse polymerase chain reaction (Inverse PCR) is a variant of the polymerase chain reaction that is used to amplify DNA with only one known sequence.
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Inverse polymerase chain reaction (Inverse PCR) is a variant of the polymerase chain reaction that is used to amplify DNA with only one known sequence. One limitation of conventional PCR is that it requires primers complementary to both termini of the target DNA, but this method allows PCR to be carried out even if only one sequence is ...
- Reverse transcription polymerase chain reaction - Wikipedia
Reverse transcription polymerase chain reaction (RT-PCR) is...
- Polymerase chain reaction - Wikipedia
The polymerase chain reaction (PCR) is a method widely used...
- Reverse transcription polymerase chain reaction - Wikipedia
Inverse Polymerase Chain Reaction. 87,123 An inverse PCR analysis uses knowledge of the sequence of the inserted plasmid, which contains a blasticidin resistance cassette (bsr), to isolate part of the genomic DNA at the REMI insertion site. From: Molecular-Genetic and Statistical Techniques for Behavioral and Neural Research, 2018
Feb 1, 2019 · The standard polymerase chain reaction (PCR) is used to amplify a segment of DNA that lies between two inward-pointing primers. In contrast, inverse PCR (also known as inverted or inside-out PCR) is used to amplify DNA sequences that flank one end of a known DNA sequence and for which no primers are ….
- Michael R. Green, Joseph Sambrook
- 2019
Inverse PCR. Inverse PCR allows unknown sequences to be amplified by PCR provided that they are located next to DNA in which the sequence is already known. The DNA is cut with a restriction enzyme that does not cut within the region of known sequence, as shown in Step 1.
Inverse PCR is a variant of PCR in which unknown DNA sequence is amplified using known DNA sequence-specific primers. Sources. Single Specific Primer-Polymerase Chain Reaction (SSP-PCR) and Genome Walking. Using an Inverse PCR Strategy to Clone Large, Contiguous Genomic DNA Fragments.
