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  1. Isabella of Portugal (Isabel in Portuguese and Spanish) (1428 – 15 August 1496) was Queen of Castile and León as the second wife of King John II. She was the mother of Queen Isabella I of Castile.

  2. Born on October 24, 1503, in Lisbon; died on May 1, 1539, in Toledo; daughter of Manuel I the Fortunate (1469–1521), king of Portugal (r. 1495–1521), and his second wife Maria of Castile (1482–1517); married Charles V (1500–1558), king of Spain (r. 1516–1556), king of the Romans (r. 1519–1530), Holy Roman Emperor (r. 1519–1558), on March 10, 152...

  3. Feb 1, 2019 · Isabella of Portugal Facts. Known for: regent of Spain during long absences of her husband, Charles V, Holy Roman Emperor. Titles: Empress, Holy Roman Empire; Queen of Germany, Spain, Naples, and Sicily; Duchess of Burgundy; princess (Infanta) of Portugal. Dates: October 24, 1503 - May 1, 1539.

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