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Congo red stain is the gold standard for the demonstration of amyloid in tissue sections. Used to evaluate the presence and extent of amyloidosis in different organs. Common diseases for Congo red stain include primary amyloidosis, amyloid light chain (AL) seen in plasma cell dyscrasias, amyloid A (AA) associated with inflammatory conditions.
Congo red is an organic compound, the sodium salt of 3,3′- ( [1,1′-biphenyl]-4,4′-diyl)bis (4-aminonaphthalene-1-sulfonic acid). It is an azo dye. Congo red is water-soluble, yielding a red colloidal solution; its solubility is greater in organic solvents.
Jan 1, 2019 · Staining with Congo Red (CR) is a qualitative method used for the identification of amyloids in vitro and in tissue sections. However, the drawbacks and artefacts obtained when using this dye can be found both in vitro and in vivo.
Although Congo red stain remains at the forefront of testing in the diagnosis of amyloidosis, its interpretation may be challenging in certain instances involving genuine cases of amyloidosis that show weak congophilia, as well as rare cases of FGN that may be also weakly congophilic.
Jan 9, 2022 · Congo red stain is the gold standard for the demonstration of amyloid in tissue sections. It is used to evaluate the presence and extent of amyloid deposits in different organs.
Congo Red and Thioflavin S are the two major histological stains used to detect any form of amyloid. These dyes bind to the characteristic β-pleated sheet conformation of amyloid.
Since its introduction as a histological stain by Bennhold (1922), Congo red staining of amyloid producing green birefringence when viewed under polarized light has become the diagnostic gold standard for this disorder.
Jul 16, 2023 · In addition to amyloid Congo Red will also stain elastin and collagen Sections cut less than 5 microns may exhibit false positive staining. It is important to align the thickness with the labs protocol and it is typically recommended to remain between 8-10 microns.
Aug 24, 2023 · Congo red (CR) stain on renal biopsy samples is the gold standard for diagnosis, but the need for polarized light is limiting the digitization of this nephropathology field. This study explores the feasibility and reliability of CR fluorescence on virtual slides (CRFvs) in evaluating the diagnostic accuracy and proposing an automated digital ...
Oct 18, 2012 · Congo red stain and electron microscopy are helpful to discriminate between amyloid and other pathologic fibrils. Once amyloid is confirmed, typing should be performed.
