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  2. (b) John Needham, who argued that microbes arose spontaneously in broth from alife force.” (c) Lazzaro Spallanzani, whose experiments with broth aimed to disprove those of Needham. Describe the theory of spontaneous generation and some of the arguments used to support it.

  3. (c) Lazzaro Spallanzani, whose experiments with broth aimed to disprove those of Needham. Describe the theory of spontaneous generation and some of the arguments used to support it. Explain how the experiments of Redi and Spallanzani challenged the theory of spontaneous generation.

    • Aristotle’s Work
    • Early Experiments
    • Disproving The Theory

    According to Aristotle, every living being is made up of a compound of matter and form. In his sexual theory of reproduction, he stated that male’s semen was efficient cause that passed down characteristics to female matter (menstrual blood), and gave rise to its offspring. He believed that the male semen and female matter were refinements that wer...

    Franceso Redi

    Franceso Redi was an Italian naturalist who challenged the ancient belief of spontaneous generation of maggots on decaying meat in 1668. He believed that maggots could be prevented if flies were not allowed direct contact with the meat. He designed an experiment where he put pieces of meat in six different containers. He covered two of them with gauze, two tightly sealed with corks and left the remaining two open in the air. His hypothesis came true as it was observed that there were no maggo...

    Pier Antonio Micheli

    Pier Antonio Micheli, an Italian botanist, performed another experiment in 1729 where he placed fungal spores on a slice of melon and observed that the same was produced on the melon slice. He concluded that the new spores definitely did not arise from spontaneous generation.

    John Needham

    John Needham, an English biologist, did yet another experiment in 1745 with boiled broths. He infused a broth by mixing plant and animal matter and boiled it in the belief that it would kill all the microorganisms. He sealed the broth and left it for a few days. He observed that the broth had become cloudy and that it has microscopic organisms in it. He reiterated the spontaneous generation theory and many of his peers believed him. However, in reality, the broth was not boiled vigorously so...

    Louis Pasteur

    In 1859, Louis Pasteur, a French microbiologist conducted another broth experiment that settled the question of spontaneous generation once and for all. He took swan flasks that had twisted necks for the experiment and boiled meat broth in it. The design of the flask was such that it allowed exchange or air from outside to inside but prevented the entry of microorganisms. If any microbes were to enter the flask they would get caught in the twisted neck of the flask. The broth remained clear f...

    John Tyndall

    John Tyndall, an Irish physicist, advanced the work of Louis Pasteur and finally the theory of spontaneous generation was disproved. Not much is known about Tyndall’s experiment on spontaneous generation. In 1862, the French Academy of Sciences, announced a prize for the scientists who shed new light on the spontaneous generation controversy and appointed a jury to decide the winner. Louis pasteur was awarded the Alhumbert Prize from the Paris Academy of Sciences for his work that totally thr...

  4. time Spallanzani decided to retest abiogenesis, scientists realized that germs or infusorial animalcules existed and could only be seen with the aide of a microscope. Spallanzani sought to test whether Redi’s hypothesis was true for these microscopic germs if a broth was boiled to kill all of the

  5. John Needham adds chick broth to a flask and boils it, lets it cool and waits. Microbes grow and he proposes it as an example of spontaneous generation. 1768: Lazzaro Spallanzani repeats Needham's experiment, but removes all the air from the flask. No growth occurs. 1859: Louis Pasteur's swan-neck flasks show that spontaneous generation does ...

  6. To find out, Spallanzani performed his own experiment. He boiled broth in two bottles, left one bottle open and one closed, and found that new microorganisms appeared only in the open bottle. His conclusion: the microorganisms entered the bottle through the air; they were not generated spontaneously in the broth (Figure 4b).

  7. Jan 30, 2022 · The experiments of Needham appeared irrefutable until the Italian physiologist Lazzaro Spallanzani repeated them and obtained conflicting results. He published his findings around 1775, claiming that Needham had not heated his tubes long enough, nor had he sealed them in a satisfactory manner.

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