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Jan 4, 2022 · Incubate the Brilliant Green Agar plate for 18-24 hours at 35°C. Examine the plates and identify suspect colonies using differential tests. If no non-lactose fermenters are observed on the primary plate cultures, inoculate Brilliant Green Agar and other media with the enrichment cultures – then proceed as in point 3. Examination of foods
Mar 7, 2024 · In conclusion, Brilliant Green Agar (BGA) is a valuable tool in microbiology for the selective isolation and identification of Salmonella species and certain other Gram-negative bacteria. Its selective properties make it particularly useful in clinical, food safety, and environmental testing, but it should be used with an awareness of its ...
Brilliant Green Agar (BGA) is a specialized culture medium widely used in microbiology, particularly for isolating and identifying Salmonella species (except S. Typhi and S. Paratyphi). Its distinct composition and features make it an invaluable resource in clinical and food microbiology contexts.
The current formulation incorporates phenol red as the pH indicator and brilliant green as an inhibitory agent that acts against gram-positive organisms and gram-negative bacilli. Organisms that ferment lactose and/or sucrose exhibit yellow to yellow-green colonies surrounded by a yellow-green zone. Salmonella appears as red to pink-white ...
Heat with frequent agitation and boil for one minute to completely dissolve the medium. Cool to 45-50°C. DO NOT AUTOCLAVE. Previous Item Number. Product was previously known as Acumedia Product: 9212 Brilliant Green Agar; and LabM Product: LAB034 Brilliant Green Agar (Modified) Package Dimensions. 4.60 in x 2.90 in x 8.60 in.
- Microbiology
- Neogen® Culture Media
Brilliant Green Agar is a highly selective medium for the isolation of Salmonella other than S. typhi from feces and other materials. Brilliant Green Agar with addition of Novobiocin confers an additional measure of selectivity by inhibiting contaminant organisms commonly seen on agar media when isolating Salmonella.
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Preparation. Suspend 58 g of the medium in one liter of purified water. Mix thoroughly. Autoclave at 121°C for 15 minutes. Cool to 45-50°C. Previous Item Number. Product was previously known as Acumedia Product: 7117 Brilliant Green Agar. Package Dimensions. 10.20 in x 10.20 in x 11.90 in.
