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- The two genetically identical daughter cells are called clones. All the progeny of a single original cell form a mass of cells on a solid surface such as agar that is called a colony. If the original form was not a single cell, for example, it was a chain of cocci, that entire chain of cells and all its progeny will form a single colony.
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What is bacterial colony morphology?
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On solid media, a colony is theoretically derived from a single cell. If well separated from other colonies, a colony will have a characteristic shape (both in elevation and margin), size, color, and consistency. Observation is often made with the naked eye, but dissecting microscopes are also used.
A colony is defined as a visible mass of microorganisms all originating from a single mother cell, therefore a colony constitutes a clone of bacteria all genetically alike. In the identification of bacteria and fungi much weight is placed on how the organism grows in or on media.
Introduction. Colony morphology is the visual culture characteristics of a bacterial colony on an agar plate. Observing colony morphology is an important skill used in the microbiology laboratory to identify microorganisms.
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- Colony Shape
- Size of The Bacterial Colony
- Appearance of The Colony Surface
- Consistency/Texture
- Color of The Colonies
- The Opacity of The Bacterial Colony
- Some Important Terminologies
It includes the form, elevation, and margin of the bacterial colony. Form of the bacterial colony:The form refers to the shape of the colony. These four forms represent the most common colony shapes you are likely to encounter. 1. circular, 2. irregular, 3. filamentous, and 4. rhizoid Elevation of the bacterial colony:It gives information about how...
The size of the colony can be a useful characteristic for identification. The diameter of a representative colony may be measured in millimeters or described in relative terms such as pinpoint, small, medium, and large. Tiny colonies are also referred to as punctiform(pin-point). Colonies larger than about 5 mm are likely to be motile organisms. Pu...
Bacterial colonies are frequently shiny and smooth in appearance. Other surface descriptions might be: dull (opposite of glistening), veined, rough, wrinkled (or shriveled), or glistening. Bacillus species give dry, wrinkled colonies. Pseudomonas stutzerialso gives similar-appearing wrinkled colonies.
Several terms that may be appropriate for describing the texture or consistency of bacterial growth are: dry, moist, viscid (sticks to loop, hard to get off), brittle/friable (dry, breaks apart), mucoid (sticky, mucus-like).
Some bacteria produce pigment when they grow in the medium, e.g., green pigment produces by Pseudomonas aeruginosa, buff-colored colonies of Mycobacterium tuberculosis in L.J medium, and red-colored colonies of Serratia marcescens.
The opacity of a bacterial colony can be described as transparent (clear), opaque (not transparent or clear), translucent (almost clear, but distorted vision–like looking through frosted glass), or iridescent (changing colors in reflected light). A pinpoint translucent β-hemolytic colonies on blood agar is most probably a Streptococcus species. Sta...
Draughtsman colonies Young colonies of Streptococcus pneumoniae (pneumococci)have raised centers, but as the culture ages, they become flattened, with a depressed central part and raised edges giving them a ringed appearance also known as ‘draughtsman colonies’. References 1. Forbes, S., Sahm, D. F., & Weissfeld, A. S. (2002). Bailey & Scott’s Diag...
Mar 14, 2024 · Apply correct terminology regarding microbial growth, when making observations. On agar plates, bacteria grow in collections of cells called colonies. Each colony arises from a single bacterium or a few bacteria (CFU).
Nov 19, 2011 · Classical microbiology uses both gross and microscopic morphology to identify microbes. Gross morphology includes colony shape, size, and surface features (Fig. 2.1). For example, Bacillus atropheus strain globigii produces an orange-pigmented colony on tryptic soy agar but produces small white colonies on other media. The structures assigned ...
On agar plates, bacteria grow in collections of cells called colonies. Each colony arises from a single bacterium or a few bacteria (CFU). Although individual cells are too small to be viewed with the unaided eye, masses of cells can be observed. Colonies can have different forms, margins, elevations and colors.
