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  1. Jul 14, 2014 · There are several factors affecting the detection of Entamoeba spp by microscopy which are lack of adequate training in microscopy, delay in delivery of the sample to the laboratory leading to degradation/death of active trophozoite forms, difficulty in differentiation of cyst with degenerated polymorphonuclear cells particularly the mature ...

  2. Apr 17, 2023 · There are two forms E. histolytica can take: the cyst form, which can survive in the environment for a prolonged period, and the trophozoite stage, which is the active and invasive form. After the ingestion of the cyst form, trophozoites can be formed, which can invade and penetrate intestinal mucosa destroying epithelial cells and inflammatory ...

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  4. Jun 3, 2020 · Entamoeba histolytica can invade multiple tissues. Trophozoites residing in the colon disrupt the intestinal barrier and subsequently disseminate via the afferent blood flow of the portal vein to reach distant sites such as liver, lung, heart, or brain.

    • Eileen Uribe-Querol, Carlos Rosales
    • 2020
  5. Aug 19, 2019 · Entamoeba histolytica trophozoites encounter cytotoxic reactive oxygen and nitrogen species in host tissues. A transcriptome study using whole-genome microarray was done with trophozoites exposed for 60 min to H 2 O 2 or to a NO donor (DPTA-NON-Oate) ( Vicente et al., 2009 ).

  6. Aug 1, 2012 · Excystation occurs in the bowel lumen and the trophozoite, the motile and disease-causing form of Entamoeba, is released [ 2 ]. While most infections are asymptomatic, about 10% of cases result in invasive disease and in rare cases (<1%, mostly adult males) E. histolytica also causes extra intestinal diseases, such as liver abscesses [ 2, 4, 5 ].

    • Laura Morf, Upinder Singh
    • 2012
  7. Nov 12, 2020 · Fact 2: Trophozoites can initiate infection in rodent models. A robust way to culture trophozoites has been developed in the laboratory setting [ 13] and has been the primary resource to perform in vivo infection. Trophozoites are surgically delivered into the target organ within the animal models.

  8. Feb 13, 2017 · Infection with E. histolytica may be the cause of a variety of symptoms, beginning from no symptoms to severe fulminating intestinal and/or life-threatening extraintestinal disease. There are at least six species of the genus Entamoeba that can be identified in human stools.