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  1. Jul 14, 2014 · There are several factors affecting the detection of Entamoeba spp by microscopy which are lack of adequate training in microscopy, delay in delivery of the sample to the laboratory leading to degradation/death of active trophozoite forms, difficulty in differentiation of cyst with degenerated polymorphonuclear cells particularly the mature ...

    • Causal Agents
    • Life Cycle
    • Geographic Distribution

    Several protozoan species in the genus Entamoeba colonize humans, but not all of them are associated with disease. Entamoeba histolytica is well recognized as a pathogenic ameba, associated with intestinal and extraintestinal infections. Other morphologically-identical Entamoeba spp., including E. dispar, E. moshkovskii, and E. bangladeshi, are gen...

    Cysts and trophozoites are passed in feces . Cysts are typically found in formed stool, whereas trophozoites are typically found in diarrheal stool. Infection with Entamoeba histolytica (and E.dispar) occurs via ingestion of mature cysts from fecally contaminated food, water, or hands. Exposure to infectious cysts and trophozoites in fecal matter d...

    Pathogenic Entamoeba species occur worldwide and are frequently recovered from fresh water contaminated with human feces. The majority of amebiasis cases occur in developing countries. In industrialized countries, risk groups include men who have sex with men, travelers, recent immigrants, immunocompromised persons, and institutionalized population...

  2. Apr 17, 2023 · There are two forms E. histolytica can take: the cyst form, which can survive in the environment for a prolonged period, and the trophozoite stage, which is the active and invasive form. After the ingestion of the cyst form, trophozoites can be formed, which can invade and penetrate intestinal mucosa destroying epithelial cells and inflammatory ...

  3. A PCR solution hybridization enzyme-linked immunoassay targeting extrachromosomal circular DNA from E. histolytica and E. dispar with specific primers and a biotin-conjugated probe was shown to be sensitive for detection and differentiation of the two Entamoeba species in clinical samples ( 7, 11, 199 ).

    • R. Fotedar, D. Stark, N. Beebe, D. Marriott, J. T. Ellis, J. Harkness
    • 10.1128/CMR.00004-07
    • 2007
    • Clin Microbiol Rev. 2007 Jul; 20(3): 511-532.
  4. Oct 6, 2022 · Clinical Findings. Laboratory diagnosis. Mode of transmission. Feco-oral route, via the ingestion of contaminated food or water containing mature quadrinucleate cyst of Entamoeba histolytica . Trophozoites if ingested would not survive exposure to the gastric environment. Mneomonic : EntAmoeba HistoLytica.

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  6. Nov 12, 2020 · E. histolytica is the causative agent of the diarrheal disease known as amebiasis, but it can sometimes penetrate the intestinal wall, enter the circulation, and cause abscesses throughout the body, most commonly in the liver. E. histolytica has 2 main stages during its life cycle: the trophozoite and the cyst stage.

  7. Feb 13, 2017 · The nonpathogenic ameba feeds on bacterial microbiota and intestinal contents. In fresh-stool examined under the microscope, the trophozoite moves actively by a finger-like protrusion of the ectoplasm “pseudopodium,” into which the cytoplasm is pulled moving the whole body of the organism in its direction.

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