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  1. Jul 14, 2014 · There are several factors affecting the detection of Entamoeba spp by microscopy which are lack of adequate training in microscopy, delay in delivery of the sample to the laboratory leading to degradation/death of active trophozoite forms, difficulty in differentiation of cyst with degenerated polymorphonuclear cells particularly the mature ...

    • Causal Agents
    • Life Cycle
    • Geographic Distribution

    Several protozoan species in the genus Entamoeba colonize humans, but not all of them are associated with disease. Entamoeba histolytica is well recognized as a pathogenic ameba, associated with intestinal and extraintestinal infections. Other morphologically-identical Entamoeba spp., including E. dispar, E. moshkovskii, and E. bangladeshi, are gen...

    Cysts and trophozoites are passed in feces . Cysts are typically found in formed stool, whereas trophozoites are typically found in diarrheal stool. Infection with Entamoeba histolytica (and E.dispar) occurs via ingestion of mature cysts from fecally contaminated food, water, or hands. Exposure to infectious cysts and trophozoites in fecal matter d...

    Pathogenic Entamoeba species occur worldwide and are frequently recovered from fresh water contaminated with human feces. The majority of amebiasis cases occur in developing countries. In industrialized countries, risk groups include men who have sex with men, travelers, recent immigrants, immunocompromised persons, and institutionalized population...

  2. A PCR solution hybridization enzyme-linked immunoassay targeting extrachromosomal circular DNA from E. histolytica and E. dispar with specific primers and a biotin-conjugated probe was shown to be sensitive for detection and differentiation of the two Entamoeba species in clinical samples ( 7, 11, 199 ).

    • R. Fotedar, D. Stark, N. Beebe, D. Marriott, J. T. Ellis, J. Harkness
    • 10.1128/CMR.00004-07
    • 2007
    • Clin Microbiol Rev. 2007 Jul; 20(3): 511-532.
  3. Apr 17, 2023 · There are two forms E. histolytica can take: the cyst form, which can survive in the environment for a prolonged period, and the trophozoite stage, which is the active and invasive form. After the ingestion of the cyst form, trophozoites can be formed, which can invade and penetrate intestinal mucosa destroying epithelial cells and inflammatory ...

    • Arthur Chou, Richard L. Austin
    • 2023/04/17
  4. Oct 6, 2022 · Life cycle of Entamoeba histolytica has two-stage: motile trophozoite and non-motile cyst. Trophozoites are found in intestinal lesions, extra-intestinal lesions, and diarrheal stools whereas cyst predominates in non-diarrheal stools.

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  6. Aug 19, 2019 · Entamoeba histolytica trophozoites encounter cytotoxic reactive oxygen and nitrogen species in host tissues. A transcriptome study using whole-genome microarray was done with trophozoites exposed for 60 min to H 2 O 2 or to a NO donor (DPTA-NON-Oate) ( Vicente et al., 2009 ).

  7. Aug 1, 2012 · Excystation occurs in the bowel lumen and the trophozoite, the motile and disease-causing form of Entamoeba, is released [ 2 ]. While most infections are asymptomatic, about 10% of cases result in invasive disease and in rare cases (<1%, mostly adult males) E. histolytica also causes extra intestinal diseases, such as liver abscesses [ 2, 4, 5 ].

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