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- A mature cyst has 4 nuclei while an immature cyst may contain only 1 to 3 nuclei.
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Mature Entamoeba histolytica/Entamoeba dispar cysts have 4 nuclei that characteristically have centrally-located karyosomes and fine, uniformly distributed peripheral chromatin. Cysts usually measure 12 to 15 µm.
Oct 6, 2022 · Cyst size and the number of its nuclei. Mature cysts of E. histolytica are smaller than those of Entamoeba coli and contain four nuclei, whereas E. coli cysts have eight nuclei. B. Antigen detection : detection of E.histolytica antigen in the stool
Apr 17, 2023 · Entamoeba histolytica is a protozoan that causes intestinal amebiasis as well as extraintestinal manifestations. Although 90 percent of E. histolytica infections are asymptomatic, nearly 50 million people become symptomatic, with about 100,000 deaths yearly.[1] Amebic infections are more prevalent in countries with lower socioeconomic conditions.[2]
- Arthur Chou, Richard L. Austin
- 2023/04/17
Entamoeba histolytica and Entamoeba dispar are morphologically identical species. In bright-field microscopy, E. histolytica/E. dispar cysts are spherical and usually measure 12 to 15 μm (range may be 10 to 20 μm). A mature cyst has 4 nuclei while an immature cyst may contain only 1 to 3 nuclei.
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Cysts range from 10 to 16 μm in diameter (mean, 12.5 μm) and contain up to 4 nuclei, each with a central irregular dot and peripheral rim of chromatin. As seen above, all 4 nuclei are rarely visible in the same plane of focus.
1 Cysts with 4 nuclei (i.e., metacysts) are ingested orally with contaminated food or drinking water ( A–C). 2–4 After excysting in the small intestine, both the cytoplasm and nuclei divide to form 8 small amoebulae (i.e., metacystic trophozoites ).
