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Aug 14, 2023 · The Gram staining is one of the most crucial staining techniques in microbiology. It gets its name from the Danish bacteriologist Hans Christian Gram, who first introduced it in 1882, mainly to identify organisms causing pneumonia. [1]
- Development of a standardized Gram stain procedure for ...
In this study, using an automated staining instrument, a...
- Gram Staining - PubMed
PMID: 32965827. Bookshelf ID: NBK562156. Excerpt. The Gram...
- Gram Staining: a Comparison of Two Automated Systems and ...
Various Gram staining automated systems are available to...
- Development of a standardized Gram stain procedure for ...
- Background
- Methods
- Results and Discussion
- Conclusions
In addition to metabolic functions of skin, the immune and protective capabilities of skin aid in separating the body’s external and internal environments . Cutaneous tissue is the first physical barrier preventing microbial invasion of underlying host tissues. When this barrier becomes compromised (e.g., after burn injury), bacteria have the oppor...
Bacterial isolates and in vitro growth conditions
A methicillin-resistant S. aureus clinical burn isolate was selected from strain USA300 at the US Army Institute of Surgical Research (JBSA Fort Sam Houston, TX, USA) collected as a part of patient care, but unrelated to research. P. aeruginosa strain PA01 is a well characterized wound isolate widely used as a laboratory strain [27–29]. S. aureus and P. aeruginosa were grown at 37 °C on blood agar plates (Remel, Lenexa, KS), and single colonies from blood agar were then inoculated into cation...
Tissue collection and processing
This study has been conducted in compliance with the Animal Welfare Act, the Implementing Animal Welfare Regulations, and the principles of the Guide for the Care and Use of Laboratory Animals. Animal protocol A14-016 was approved by the Institutional Animal Care and Use Committee for United States Army Institute of Surgical Research. Skin biopsies from day 11 post-burn were kindly provided from a previously described porcine burn infected model currently under review . Additionally, huma...
Staining techniques
Antibody specificity for S. aureus and P. aeruginosa was tested in vitro by hydrating the biofilm or planktonic smears with Hank’s balanced salt solution (HBSS). Smears were then blocked with 1 % bovine serum albumin (BSA) in tris-buffered saline (TBS) with 0.1 % Tween 20 and 0.1 % Triton X-100 for 1 h at room temperature. Smears were then incubated with the following primary antibodies for 1 h at room temperature: S. aureus-FITC (1:50 dilution in HBSS, ab68950, Abcam, Cambridge, UK), or P. a...
Gram stain is the most common staining technique used diagnostically within both the clinical setting and in research laboratories to differentiate between Gram positive and Gram negative microorganisms in various types of tissues [17, 26]. As seen in Fig. 2, in vitro Gram stain of S. aureus and P. aeruginosa readily differentiates between these tw...
We have described a histological technique that allows for visualization of tissue structures along with detection of Gram positive and Gram negative bacteria. An important advantage to the modified Gram stain is its ability to highlight collagen, making it applicable to any tissues that contain collagen. Employing this technique in other collagen-...
- Sandra C. Becerra, Daniel C. Roy, Carlos J. Sanchez, Robert J. Christy, David M. Burmeister
- 2016
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PDF | Gram staining is an empirical method of differentiating bacterial species into two large groups (Gram-positive and Gram-negative) based on the... | Find, read and cite all the research...
Nov 4, 2016 · EDITORIAL. From the teaching laboratory to the clinical microbiology laboratory, one of the best-known tools to study microbes is the Gram stain. This stain, originally developed to help distinguish bacteria from host cells in tissue, has evolved as a key assay to help clinicians decide which antibiotics should be used to treat infections ...
