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  1. Type and morphology. E. coli is a gram-negative, facultative anaerobe, nonsporulating coliform bacterium. Cells are typically rod-shaped, and are about 2.0 μm long and 0.25–1.0 μm in diameter, with a cell volume of 0.6–0.7 μm 3.

    • EPEC

      Escherichia coli (/ ˌ ɛ ʃ ə ˈ r ɪ k i ə ˈ k oʊ l aɪ /...

    • Gram-negative

      Microscopic image of gram-negative Pseudomonas aeruginosa...

    • Enterobacteriaceae

      Enterobacteriaceae is a large family of Gram-negative...

  2. Dec 31, 2022 · Escherichia coli have served as important model organisms for over a century—used to elucidate key aspects of genetics, evolution, molecular biology, and pathogenesis. However, defining which strains actually belong to this species is erratic and unstable due to shifts in the characters and criteria used to distinguish bacterial species.

    • Marta Cobo-Simón, Rowan Hart, Howard Ochman
    • Mol Biol Evol. 2023 Jan; 40(1): msac273.
    • 10.1093/molbev/msac273
    • 2023/01
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  4. Aug 31, 2023 · Summary. There are three basic shapes of bacteria: coccus, bacillus, and spiral. Based on planes of division, the coccus shape can appear in several distinct arrangements: diplococcus, streptococcus, tetrad, sarcina, and staphylococcus. The bacillus shape can appear as a single bacillus, a streptobacillus, or a coccobacillus.

  5. Jan 1, 2008 · E. coli is Gram-negative and its envelope has three layers: cytoplasmic membrane, peptidoglycan, and outer membrane. The peptidoglycan is rigid determining the rod shape. To a good approximation, the E. coli cell has hemispherical caps and a cylindrical section in between.

    • Galina Reshes, Sharon Vanounou, Itzhak Fishov, Mario Feingold
    • 10.1529/biophysj.107.104398
    • 2008
    • Biophys J. 2008 Jan 1; 94(1): 251-264.
    • Diseases Caused by E. Coli
    • Characteristics of E. Coli
    • Virulence Factors of E.coli
    • Diagnostic Features of E.coli
    • Antimicrobial Susceptibility Testing
    E.coli is the second most important cause of Gram-negative rod sepsis
    Perinatal infection with E.coli (exposure of newborn to E.coli colonized in the birth canal of the mother during natural birth) is the predominant cause of neonatal meningitis
    Enterotoxigenic Escherichia coli (ETEC) is responsible for the traveler’s diarrhea(watery diarrhea)
    Gram stain: Escherichia coliis a straight gram-negative short rod or bacilli
    Escherichia coli cells are small rods 1.0-2.0 micrometers long, with a radius of about 0.5 micrometers. However, the size varies with the medium, and faster-growing cells are larger.
    E. coliis the most abundant facultative anaerobe in the colon and feces.
    The generation (doubling) time of Escherichia coliis 20 minutes.
    Pili:Helps in adherence of organisms to the cells of jejunum and ileum in case of intestinal tract infection; urinary tract epithelium in case of urinary tract infections.
    Endotoxin (lipopolysaccharide): Responsible for several features of gram-negative sepsis such as fever, hypotension, and disseminated intravascular coagulation (DIC).
    Exotoxinse.g. enterotoxin act on the cells of the jejunum and ileum to cause diarrhea. Other exotoxins are verotoxin, Shiga-like toxin, etc.
    Colony Morphology: E.coli ferments lactose and produces pink colonies on MacConkey Agar. Typical colonies of Escherichia coli on MacConkey agar will appear pink and shiny and have a diameter of 0.5...
    On EMB agar, E. coliproduces a characteristic green sheen.

    Perform the susceptibility test by a disc-diffusion method using standard methods described in the guidelines. To detect ESBL-producing E.coli, the isolate screened should be multidrug-resistant exhibiting resistance to at least one of the third-generation cephalosporins. Extended-spectrum ß-lactamases (ESBL) are a rapidly evolving group of ß-lacta...

  6. Aug 31, 2023 · Escherichia coli. Gram Stain of Escherichia coli. Note gram-negative (pink) bacilli. Escherichia coli is shared under a CC BY license and was authored, remixed, and/or curated by LibreTexts.

  7. Feb 13, 2021 · Coliforms have historically been an important group of bacteria in food microbiology, for their practical role in serving as indicator organisms for possible faecal contamination and even E. coli contamination. This piece of writing was completed by Suryani Tan, one of my 2nd-year food microbiology students at The University of Melbourne in 2007.