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  1. Jan 4, 2022 · Uses of Brilliant Green Agar. Brilliant Green Agar is a selective and differential medium for the isolation of Salmonella species other than S. Typhi and S. Paratyphi from clinical specimens. I t is also recommended by APHA FDA and is in accordance with United States Pharmacopoeia. This medium is employed in testing clinical specimens.

  2. Mar 7, 2024 · Brilliant Green Agar (BGA) is a specialized microbiological growth medium used in laboratories for the isolation and differentiation of Gram-negative bacteria, particularly members of the Salmonella genus. It is a selective and differential agar that helps in the identification of Salmonella species from other bacteria.

  3. The substrate for decarboxylase is lysine. The pH indicator is called phenol red. With the exception of Salmonella species, brilliant green is the selective agent that inhibits Gram-positive and the majority of Gram-negative bacteria. The solidifying agent is agar. Preparation of BGA Materials Needed. Brilliant Green Agar powder: 58.09 grams

  4. The current formulation incorporates phenol red as the pH indicator and brilliant green as an inhibitory agent that acts against gram-positive organisms and gram-negative bacilli. Organisms that ferment lactose and/or sucrose exhibit yellow to yellow-green colonies surrounded by a yellow-green zone. Salmonella appears as red to pink-white ...

  5. Brilliant Green Sulfa Agar is recommended by the American Public Health Association (APHA) and the U.S. Department of Agriculture (USDA) to isolate Salmonella from foods, eggs, and meat products.3,4 PRINCIPLE Casein and meat peptones provide nitrogen, amino acids, and peptides necessary for bacterial growth. Yeast extract is a source of B-complex

  6. BRILLIANT GREEN AGAR. - For in vitro use only - Catalogue No. PB84. Our Brilliant Green Agar is a selective and differential medium for the isolation of Salmonella species other than S. typhi and S. paratyphi from clinical specimens. Brilliant Green Agar was initially developed in 1925 by Kristensen and later modified by Kauffmann in 1935.

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  8. In 1925, Kristensen et al. described brilliant green agar for isolation of Salmonella spp.1 Kauffmann reported increased isolation rates for Salmonella spp. from fecal specimens using a modification of Kristensen’s formula.2 Devenish and Cooke, in separate studies, added novobiocin to brilliant green agar to further improve selectivity.3,4

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