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In the field of genetic sequencing, genotyping by sequencing, also called GBS, is a method to discover single nucleotide polymorphisms (SNP) in order to perform genotyping studies, such as genome-wide association studies ( GWAS ). [1] GBS uses restriction enzymes to reduce genome complexity and genotype multiple DNA samples. [2]
Genotyping by sequencing, or next-generation genotyping, is a genetic screening method for discovering novel plant and animal SNPs and performing genotyping studies. For some applications, such as genotype screening and genetic mapping, sequence-based genotyping provides a lower-cost alternative to arrays for studying genetic variation.
Oct 1, 2020 · Genotyping-by-Sequencing (GBS) is a low-cost, high-throughput genotyping method that relies on restriction enzymes to reduce genome complexity. GBS is being widely used for...
- Nan Wang, Yibing Yuan, Yibing Yuan, Hui Wang, Diansi Yu, Yubo Liu, Yubo Liu, Ao Zhang, Manje Gowda, ...
- 2020
Sep 29, 2014 · To further broaden NGS usages to large crop genomes such as maize and wheat, genotyping-by-sequencing (GBS) has been developed and applied in sequencing multiplexed samples that combine molecular marker discovery and genotyping. GBS is a novel application of NGS protocols for discovering and genotyping SNPs in crop genomes and populations.
In essence, GBS entails sequencing a subset of the genome using a restriction enzyme to generate a library of reduced representation fragments, which are then sequenced using next-generation sequencing (NGS) technologies.
Genotyping-by-sequencing (GBS) refers to a suite of related methods that obtain genotype data from samples by using restriction enzyme digestion followed by high-throughput sequencing. GBS is a refinement of restriction site-associated DNA sequencing (RADseq) methods, with a goal of being able to pe ….
Genotyping by sequencing (GBS) in any large genome species requires reduction of genome complexity. I. Target enrichment. Long range PCR of specific genes or genomic subsets. Molecular inversion probes. Sequence capture approaches hybridization‐based (microarrays) . II. Restriction Enzymes (REs)
