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1. www.ncbi.nlm.nih.gov › pmc › articlesWestern Blotting: An Introduction - National Center for ...

   www.ncbi.nlm.nih.gov › pmc › articles

   Western blotting is an important procedure for the immunodetection of proteins, particularly proteins that are of low abundance. This process involves the transfer of protein patterns from gel to microporous membrane. Electrophoretic as well as non-electrophoretic ...

2. media.cellsignal.com › www › pdfsmedia.cellsignal.com

   media.cellsignal.com › www › pdfs

   Here, we will highlight the critical steps in the western blot protocol and demonstrate how protocol changes can afect the final outcome of your blot. We will also discuss the importance of using a well-validated antibody in your western blot experiments. Finally, we will provide a list of commonly used reagents that are utilized by CST scientists and work optimally with our antibodies.

3. docs.abcam.com › general-western-blot-protocolGeneral western blot protocol - Abcam

   docs.abcam.com › general-western-blot-protocol

   Western blotting is used to visualize proteins that have been separated by gel electrophoresis. The gel is placed next to a nitrocellulose or PVDF (polyvinylidene fluoride) membrane and an electrical current causes the proteins to migrate from the gel to the membrane.

4. www.abcam.com › protocols › transfer-and-staining-ofTransfer and staining of proteins in western blot - Abcam

   www.abcam.com › protocols › transfer-and-staining-of

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   Detailed protocol for the transfer and staining of proteins in western blot. Includes visualization of proteins in gels, transfer, and development methods.

5. www.origene.com › protocols › antibodyprotocolWestern Blot Protocol | OriGene

   www.origene.com › protocols › antibodyprotocol

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   Western Blot Protocol. Cell Lysing Protocol: Remove the culture media by aspiration. Wash cells in the dish with ice-cold PBS and aspirate off PBS (1X). Add ice-cold NP40 Cell Lysis Buffer (RIPA Lysis Buffer: 25mM Tris-HCl pH7.5, 150mM NaCl, 1% NP-40, 1mM EDTA pH8.0. Add fresh: 1 mM PMSF, 1 mM Na3VO4, and 1 X Protease Inhibitor Cocktail-P2714 ...

6. www.abcam.com › protocols › western-blotWestern blot protocol | abcam

   www.abcam.com › protocols › western-blot

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   This protocol guides you through the western blot procedure using chemiluminescent and fluorescent detection.

7. www.abcam.com › protocols › western-blotWestern blot protocol | abcam

   www.abcam.com › protocols › western-blot

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   This protocol guides you through the western blot procedure using chemiluminescent and fluorescent detection.