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Congo red stain is the gold standard for the demonstration of amyloid in tissue sections. Used to evaluate the presence and extent of amyloidosis in different organs. Common diseases for Congo red stain include primary amyloidosis, amyloid light chain (AL) seen in plasma cell dyscrasias, amyloid A (AA) associated with inflammatory conditions.
Congo red is an organic compound, the sodium salt of 3,3′- ( [1,1′-biphenyl]-4,4′-diyl)bis (4-aminonaphthalene-1-sulfonic acid). It is an azo dye. Congo red is water-soluble, yielding a red colloidal solution; its solubility is greater in organic solvents.
Jan 9, 2022 · Congo red stain is the gold standard for the demonstration of amyloid in tissue sections. It is used to evaluate the presence and extent of amyloid deposits in different organs.
CONGO RED - PUTCHLER'S MODIFICATION - AMYLOID PURPOSE: To demonstrate amyloid deposits in tissue sections. PRINCIPLE: Amyloid is homogeneous and eosinophilic, the deposits are extracellular and may become sufficiently large enough to cause damage to surrounding tissues. when stained with the Congo Red Stain the
Since its introduction as a histological stain by Bennhold (1922), Congo red staining of amyloid producing green birefringence when viewed under polarized light has become the diagnostic gold standard for this disorder.
Jan 1, 2019 · Staining with Congo Red (CR) is a qualitative method used for the identification of amyloids in vitro and in tissue sections. However, the drawbacks and artefacts obtained when using this dye can be found both in vitro and in vivo.
Jul 16, 2023 · The biochemical properties that lead to positive staining in Congo Red are poorly understood. It is thought that a properly made, appropriately linear molecule of Congo Red has affinity for the beta pleated sheets that are the common make up of amyloid.
May 31, 2017 · David P. Steensma, MD, offers readers a fascinating history of the Congo red stain (see references), which still remains the most widely used and cost-effective way to identify amyloid deposits in pathology specimens.
Nov 7, 2022 · Our study aimed at evaluating the ability of Congo red fluorescence microscopy using the Texas Red filter to mitigate the false positivity seen with LM and thus enhance the specificity of Congo red stain in detecting amyloid deposits in histology sections from a variety of tissue types.
Congo Red Method. The main special stain used for amyloid detection is Congo Red. The reason for this is that the Congo red dye used in the stain is highly selective for amyloid deposits, which can lead to amyloidosis and also alzheimers.
