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- Optimization of the polymerase chain reaction (PCR) includes the consideration of buffer, additives, magnesium chloride, deoxynucleotides, primer design, thermostable DNA polymerase, DNA template, reaction volume, denaturation time, annealing temperature and extension time.
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The polymerase chain reaction (PCR) is a commonly used molecular biology tool for amplifying DNA, and various techniques for PCR optimization which have been developed by molecular biologists to improve PCR performance and minimize failure. Contamination and PCR.
May 22, 2012 · PCR failures can become frustrating unless patience and careful troubleshooting are employed to sort out and solve the problem (s). This protocol outlines the basic principles of PCR, provides a methodology that will result in amplification of most target sequences, and presents strategies for optimizing a reaction.
- Todd C. Lorenz
- 10.3791/3998
- 2012
- J Vis Exp. 2012; (63): 3998.
May 19, 2009 · Abstract. Real-time polymerase chain reaction (PCR) is a frequently used technique in molecular diagnostics. To date, practical guidelines for the complete process of optimization and validation of commercial and in-house developed molecular diagnostic methods are scare.
- Marijke Raymaekers, Rita Smets, Brigitte Maes, Reinoud Cartuyvels
- 2009
Mar 22, 2022 · A surrogate-enabled multi-objective optimisation methodology for a continuous flow Polymerase Chain Reaction (CFPCR) systems is presented, which enables the effect of the applied PCR protocol and the channel width in the extension zone on four practical objectives of interest, to be explored.
Jan 29, 2018 · Polymerase chain reaction (PCR)-based site-directed mutagenesis is an invaluable technique for altering genes and hence the structure and activity of individual proteins in a systematic way,...
- Fanli Zeng, Suhua Zhang, Zhimin Hao, Shixin Duan, Yanan Meng, Pan Li, Jingao Dong, Yibin Lin
- 2018
A surrogate-enabled multi-objective optimisation methodology for a continuous flow Polymerase Chain Reaction (CFPCR) systems is presented, which enables the effect of the applied PCR protocol and the channel width in the extension zone on four practical objectives of interest, to be explored.
Dec 17, 2019 · The development of multiplex polymerase chain reaction and microarray assays is challenging due to primer dimer formation, unspecific hybridization events, the generation of unspecific...
