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Slice, fix overnight. Tissue size: factors that affect fixation. Routine: 3mm thick. 4-6 hours fast penetration. 1-3 days to truly fix (cross-link) For every 4-6 hours it will penetrate 3mm deep. Electron Microscope: 1mm^3. 2-4 hours, store in buffer. For every 2-4 hours, it will penetrate 1mm^3 deep.
- Chapter 4 fixation objectives Flashcards
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- Histology Flashcards
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- Histology Fixation Review Flashcards
Define the purpose of fixation Fixation alters tissue by...
- Chapter 4 fixation objectives Flashcards
Study with Quizlet and memorize flashcards containing terms like Define the purpose of fixation ?, Autolysis, function of fixatives and more.
What are the two means of fixation? What are the steps in microwave fixation? 1. Fix tissue in saline or formalin enough so it is possible to be grossed. 2. Cut into 2mm blocks and immerse in saline or formalin and microwave to a temp of 45-68C. What kind of fixatives bind with denatured proteins?
FIXATION. Stabilizing the protein so that it is resistant to further changes. AUTOLYSIS. Destruction of tissues and cells by the enzymes. (enzymes attack) ARTIFACT. A structures or substance not normally present but produced by some external force, or action. Ex: mercury pigment, tissue floaters, knife lines, and air bubbles.
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