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Congo red stain is the gold standard for the demonstration of amyloid in tissue sections. Used to evaluate the presence and extent of amyloidosis in different organs. Common diseases for Congo red stain include primary amyloidosis, amyloid light chain (AL) seen in plasma cell dyscrasias, amyloid A (AA) associated with inflammatory conditions.
Jan 1, 2019 · Staining with Congo Red (CR) is a qualitative method used for the identification of amyloids in vitro and in tissue sections. However, the drawbacks and artefacts obtained when using this dye can be found both in vitro and in vivo.
Congo red is an organic compound, the sodium salt of 3,3′- ( [1,1′-biphenyl]-4,4′-diyl)bis (4-aminonaphthalene-1-sulfonic acid). It is an azo dye. Congo red is water-soluble, yielding a red colloidal solution; its solubility is greater in organic solvents.
Since its introduction as a histological stain by Bennhold (1922), Congo red staining of amyloid producing green birefringence when viewed under polarized light has become the diagnostic gold standard for this disorder.
CONGO RED - PUTCHLER'S MODIFICATION - AMYLOID PURPOSE: To demonstrate amyloid deposits in tissue sections. PRINCIPLE: Amyloid is homogeneous and eosinophilic, the deposits are extracellular and may become sufficiently large enough to cause damage to surrounding tissues. when stained with the Congo Red Stain the
Jan 9, 2022 · Congo red stain is the gold standard for the demonstration of amyloid in tissue sections. It is used to evaluate the presence and extent of amyloid deposits in different organs.
Although Congo red staining has long been accepted as the gold standard for amyloid identification, the interpretation of this stain is not always easy because the staining procedure requires expertise and the use of polarized light microscopy.
Congo Red and Thioflavin S are the two major histological stains used to detect any form of amyloid. These dyes bind to the characteristic β-pleated sheet conformation of amyloid.
Nov 7, 2022 · Our study aimed at evaluating the ability of Congo red fluorescence microscopy using the Texas Red filter to mitigate the false positivity seen with LM and thus enhance the specificity of Congo red stain in detecting amyloid deposits in histology sections from a variety of tissue types.
Jan 15, 2019 · Staining with Congo Red (CR) is a qualitative method used for the identification of amyloids in vitro and in tissue sections.
