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   What is a mordant in a Gram stain?

   • A mordant is a substance that increases the affinity of the cell wall for a stain by binding to the primary stain, thus forming an insoluble complex which gets trapped in the cell wall. In the Gram stain reaction, the crystal violet and iodine form an insoluble complex (CV-I) which serves to turn the smear a dark purple color.

   Gram Stain Technique (Theory) : Microbiology Virtual Lab I

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   What stains are used in microbiology?

   • Table 1: Summary of some common differential stains used in microbiology. The Gram stain uses the following dyes/reagents: crystal violet, Gram's iodine, ethanol, and safranin. The Gram stain distinguishes cells by cell wall type (Gram-positive or Gram negative). Gram-positive cells stain purple/violet. Gram-negative cells stain pink.

   1.10: Gram Stain - Biology LibreTexts

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   What is a Gram stain used for in microbiology & molecular biology?

   • In microbiology and molecular biology, it is used for staining bacteria, histological slide staining, DNA staining, etc. It also shows antibacterial and antifungal properties, hence used in sterilization and disinfection. In Gram Staining, it is used as a basic dye in the ionized form of CV+ and Cl-.

   Gram Staining- Principle, Reagents, Procedure, Steps, Results

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2. microbeonline.com › gram-staining-principleGram Staining: Principle, Procedure, Results • Microbe Online

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   Apr 21, 2024 · Application of mordant: The iodine solution (mordant) is added to form a crystal violet-iodine (CV-I) complex; all cells continue to appear blue. Decolorization step : The decolorization step distinguishes gram-positive from gram-negative cells.

3. microbenotes.com › gram-stain-principle-reagentsGram Staining- Principle, Reagents, Procedure, Steps, Results

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   Feb 4, 2022 · 1. Primary Stain (Crystal Violet) 2. Mordant (Gram’s Iodine) 3. Decolorizing Solution. 4. Counter Stain (Safranin) Procedure of Gram Staining. Gram Staining Reagents Preparation. Procedure of Gram Stain Slide Preparation. Gram Staining Protocol. Procedure of Microscopic Observation of Gram Stain. Result and Interpretation of Gram Staining.

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4. bio.libretexts.org › Bookshelves › Microbiology1.10: Gram Stain - Biology LibreTexts

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   The endospore stain is used to distinguish between bacterial species that do and do not form endospores. Endospores (if present) appear bluish-green and all other cells/cell structures appear pink. The flagella stain uses the following dyes/reagents: tannic acid or potassium alum mordant, pararosaline or basic fuchsin.

5. www.thoughtco.com › gram-stain-procedure-4147683Gram Stain Procedure in Microbiology - ThoughtCo

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   The procedure is based on the reaction between peptidoglycan in the cell walls of some bacteria. The Gram stain involves staining bacteria, fixing the color with a mordant, decolorizing the cells, and applying a counterstain. The primary stain ( crystal violet) binds to peptidoglycan, coloring cells purple. Both gram-positive and gram-negative ...

6. vlab.amrita.eduGram Stain Technique (Theory) : Microbiology Virtual Lab I ...

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   2 days ago · Principle: Staining is an auxiliary technique used in microscopic techniques used to enhance the clarity of the microscopic image.Stains and dyes are widely used in the scientific field to highlight the structure of the biological specimens, cells, tissues etc.

7. courses.lumenlearning.com › suny-microbiologyStaining Microscopic Specimens | Microbiology - Lumen Learning

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   A mordant is a substance used to set or stabilize stains or dyes; in this case, Gram’s iodine acts like a trapping agent that complexes with the crystal violet, making the crystal violet–iodine complex clump and stay contained in thick layers of peptidoglycan in the cell walls.

8. microbenotes.com › flagella-stain-principleFlagella Stain- Principle, Procedure and Result Interpretation

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   Jun 7, 2021 · The staining procedures require the use of a mordant so that the stain adheres in layers to the flagella, allowing visualization. The procedure of Flagella Stain (Wet Mount Technique) Grow the organism to be stained at room temperature on blood agar for 16 to 24 hours.