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Sep 28, 2007 · Abstract. The classification of glycosidases into families and clans on the basis of amino acid sequence has provided a foundation for the analysis of the structures and mechanisms of these important enzymes. This confluence of bioinformatic, structural and mechanistic studies has greatly advanced glycosidase engineering and the development of ...
Oct 1, 2002 · Introduction. Many essential features of the general mechanism of action of retaining and inverting glycoside hydrolases (glycosidases) are known and have been discussed in recent reviews 1., 2., 3.. Kinetic isotope effects (KIEs), crystal structure analysis of wild type (wt) and mutated glycosidases and of their complexes with ligands, and the ...
Jul 15, 2014 · Glycosidases adopt a variety of folds. The vast majority of glycosidases act via Koshland mechanisms with either retention or inversion of the anomeric configuration. Some glycosidases use unusual mechanisms employing hydration, elimination and/or redox steps. Detailed mechanistic understanding of glycosidase mechanism has allowed the ...
- Miriam P Kötzler, Susan M Hancock, Stephen G Withers
- 2007
In recent years, carbohydrate-processing enzymes have become the enzymes of choice in many applications thanks to their stereoselectivity and efficiency. This review presents recent developments in glycosidase-catalyzed synthesis via two complementary approaches: the use of wild-type enzymes with engineered substrates, and mutant glycosidases. Genetic engineering has recently produced ...
- Pavla Bojarová, Pavla Bojarová, Vladimír Křen
- 2009
Abstract. In recent years, carbohydrate-processing enzymes have become the enzymes of choice in many applications thanks to their stereoselectivity and efficiency. This review presents recent developments in glycosidase-catalyzed synthesis via two complementary approaches: the use of wild-type enzymes with engineered substrates, and mutant ...
Glycosylation produces an abundant, diverse, and highly regulated repertoire of cellular glycans that are frequently attached to proteins and lipids. The past decade of research on glycan function has revealed that the enzymes responsible for glycosylation—the glycosyltransferases and glycosidases—are essential in the development and physiology of living organisms. Glycans participate in ...
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What are the latest developments in glycosidase-catalyzed synthesis?
Are carbohydrate-processing enzymes suitable for glycosidase-catalyzed synthesis?
What are abnormal glycosidase activities?
How do glycosidases Act?
Sep 6, 2023 · Abnormal glycosidase activities are associated with a variety of diseases, particularly cancer and lysosomal storage disorders. Owing to the physiological and pathological significance of glycosidases, the development of small molecules that target these enzymes is an active area in glycoscience and medicinal chemistry.
