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Jun 1, 2021 · Here we presented a double-network cross-linked polysaccharide-based hydrogel composed of collagen peptide-functionalized carboxymethyl chitosan (CS) and oxidized methacrylate sodium alginate (SA). The hydrogel possessed interconnected porous morphologies, suitable swelling ratios, excellent mechanical properties, and favorable biocompatibility ...
- Yuxin He, Yang Li, Yadong Sun, Shijia Zhao, Miao Feng, Guoming Xu, Haofang Zhu, Peihong Ji, Hongli M...
- 2021
- Introduction
- Materials and Methods
- Results and Discussion
- Conclusion
- Data Availability Statement
- Author Contributions
- Funding
- Conflict of Interest
- Publisher’S Note
- Acknowledgments
Rapid hemostasis and effective wound closure are very important in wound repair. Conventional suturing is usually time consuming and cannot close the wound immediately, which can lead to a variety of pathologic scenarios, such as tissue morbidities and mortalities (Geng et al., 2020; Zhu et al., 2020). Sutureless wound closure, which primarily util...
Materials
Sodium alginate (low viscosity), gelatin (from porcine skin), and sodium periodate (NaIO4) were purchased from Aladdin Industrial Corporation (Shanghai, China). Dulbecco’s modified Eagle medium (DMEM) and fetal bovine serum (FBS) were purchased from Life Technologies Corporation (California, United States). A Porcine Fibrin Sealant Kit was purchased from Guangzhou Bioseal Biotech Co., Ltd. All other chemicals were purchased from Sigma-Aldrich and used as received unless specified otherwise.
Preparation of OSA
Oxidized sodium alginate was synthesized as reported previously (Yuan et al., 2018). Briefly, 3.00 g raw sodium alginate (SA) was dissolved in ultrapure water (300 ml) and 4.86 g NaIO4 was added. After that, the mixed solution was stirred for another 4 h without light, and then 5 ml ethylene glycol was added in the mixed solution to terminate the oxidation. The final product was dialyzed (MWCO = 3,500 Da) for 3 days in distilled water. Finally, the product was lyophilized. To confirm the char...
Preparation of Gel-ADH
To synthesize ADH-modified gelatin, 3.00 g gelatin and 2.40 g adipic acid dihydrazide were dissolved in 300 ml ultrapure deionized water with stirring at 55°C. Then, 0.50 g EDC and 0.50 g HOBt were added to the solution. Finally, 0.1 M HCl solution was used to adjust the pH of the abovementioned solution to 5. The mixed solution was stirred overnight. The final product was dialyzed (MWCO = 7,000 Da) for 5 days in distilled water (Hozumi et al., 2018). The 1H NMR spectra of the Gel-ADH was mea...
Synthesis and Characterization of OSA and Gel-ADH
The preparation scheme of OSA and Gel-ADH is shown in Figure 1. Figure 2A shows the FTIR spectra of SA and OSA. Compared with the spectra of SA, a new absorption peak at 1726 cm−1 which is due to the aldehyde group was observed in OSA. The oxidation degree of OSA used in this study was about 79%. The 1H NMR spectra of Gel and Gel-ADH are shown in Figure 2B. Compared with the curve of Gel, new methylene peaks of ADH at 2.07 and 1.46 ppm were found in the Gel-ADH, indicating that gelatin was su...
Characterization of the Gel-ADH/OSA Hydrogel
Without further treatment, the sol-gel transition of the Gel-ADH/OSA hydrogel occurred relatively fast, as indicated by tilt tests (Figure 3A). Meanwhile, the hydrogel adhesive developed here was completely transparent and would not cover the underlying tissues when used by surgeons. Figure 3B shows the gelation time of the Gel-ADH/OSA hydrogel with different components and concentrations. The gelation time of 10% Gel-ADH/OSA 1:1, 10% Gel-ADH/OSA 1:1, 15% Gel-ADH/OSA 1:1, and 20% Gel-ADH/OSA...
Biocompatibility of the Gel-ADH/OSA Hydrogel In Vitro
It is well known that one of the key factors hindering the wide application of biomedical adhesives is their toxic and side effects on cells or their degradation products that can lead to wound tissue infection and necrosis. To investigate the biocompatibility of the Gel-ADH/OSA hydrogel, L929 cells were employed and directly incubated with the Gel-ADH/OSA hydrogel. Then, the CCK-8 assay and live/dead staining were performed on the first and third day of incubation (Figures 4A,B). Compared to...
In this study, an injectable hydrogel tissue adhesive was developed, and the hydrogel showed a suitable swelling ratio, good injectability, and excellent biocompatibility. We also found that the adhesion capacity was related to the prepolymer component and concentration. The 20% Gel-ADH/OSA 1:1 hydrogel showed the highest adhesion ability, which wa...
The original contributions presented in the study are included in the article/Supplementary Material; further inquiries can be directed to the corresponding authors.
HM and ZG contributed to the conception and design of the study. YX, HX, and SH performed the experiments. YX wrote the first draft of the article. HX, SH, and HZ wrote sections of the article. HM, XQ, YH, and ZG performed the statistical analysis and writing—review and editing. All authors contributed to article revision, read, and approved the su...
This work was supported by the Key R&D Plan of Jiangsu Province (BE2018010-3), the National Natural Science Foundation of China (31800803, 31771067, and 32071364), and the Scientific Research Foundation for Talent Introduction of Nanjing Tech University (39803129 and 39803130).
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors, and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.
The authors gratefully acknowledge the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).
- Yuhang Xing, Xueqin Qing, Hao Xia, Shiqi Hao, Haofang Zhu, Yiyan He, Hongli Mao, Zhongwei Gu
- 2021
Jun 1, 2021 · In recent years, chitosan-based natural polysaccharide hydrogels have garnered attention for their biocompatibility, antimicrobial properties, and ability to aid in hemostasis. This study presents the development of a multi-functional, bi-dynamic network hydrogel for the treatment of wounds infected with bacteria.
- Yuxin He, Yang Li, Yadong Sun, Shijia Zhao, Miao Feng, Guoming Xu, Haofang Zhu, Peihong Ji, Hongli M...
- 2021
Injectable Hydrogel Based on Modified Gelatin and Sodium Alginate for Soft-Tissue Adhesive Yuhang Xing1†, Xueqin Qing2†, Hao Xia1†, Shiqi Hao1, Haofang Zhu1, Yiyan He1,3,4, Hongli Mao 1 ,3 ...
FACULTY IN FOCUS. Xiaohang Li. No Half Measures: With Semiconductors, the Sky is the Limit. “I see a lot of myself in the students I interact with.” – Xiaohang Li. Professor Xiaohang Li has his eyes firmly set on the future of humanity.
Aug 1, 2021 · Zhen Zhang 1, Junqiang Ma 1 *, Menghou Li 1, Haofang Li 1 1 Zhengzhou University School of Electrical Engineering, Zhengzho u 450001, China *Corresponding author’s e-mail:junqiang@gs.zzu.edu.cn
Jan 1, 2024 · The optimal extraction process of Astragali radix-Paeoniae radix alba predicted by SA-BPNN was as follows: the extraction time was 53.8 min, the extraction temperature was 50.8 °C, the NADES-water ratio was 46.5:53.5 (v/v), and the solid-liquid ratio was 163.1 mg/mL. The predicted value of SA-BPNN was 226.2 under this condition.
