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   Is Western blot test accurate and reliable to detect HIV?

   • This very sensitive test was the first one widely used to check for HIV. The Western blot test was previously used to confirm the result of the ELISA, but it is no longer recommended, as other tests are now more reliable and enable a faster diagnosis.

   Western Blot Test | Stanford Health Care

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   Why is Western blot better than ELISA?

   • •Western Blotting utilizes not only antigens, but also antisera as a diagnostic tool. Antisera is widely used in the test for HIV presence. •Compared to ELISA, Western blotting has higher specificity; the higher specificity, the more the method is independent of the specificity of antibodies.

   western blot and ELISA - ResearchGate

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   Is a positive Western blot proof of HIV infection?

   • The Western blot test separates the blood proteins and detects the specific proteins (called HIV antibodies) that indicate an HIV infection. The Western blot is used to confirm a positive ELISA, and the combined tests are 99.9% accurate.

   Western Blot Test | Stanford Health Care

   stanfordhealthcare.org/medical-conditions/sexual-and-reproductive-health/hiv-aids/diagnosis/western-blot-test.html

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2. www.healthline.com › health › hivWestern Blot Test: Uses, Accuracy, and More - Healthline

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   May 3, 2021 · The Western blot test is used to confirm or contest a diagnosis of HIV or Lyme disease after an ELISA antibody test comes back positive or negative. Since the ELISA test sometimes...

   • Author: Corey Whelan

3. stanfordhealthcare.org › western-blot-testWestern Blot Test | Stanford Health Care

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   The Western blot test separates the blood proteins and detects the specific proteins (called HIV antibodies) that indicate an HIV infection. The Western blot is used to confirm a positive ELISA, and the combined tests are 99.9% accurate.

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5. www.verywellhealth.com › how-does-a-western-blotHow Does a Western Blot HIV Test Work? - Verywell Health

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   Jan 21, 2023 · For an HIV Western blot test, scientists work with prepared samples of HIV proteins and look to see if there are any antibodies in a person's blood that stick to them.

6. www.medicalnewstoday.com › articles › 316230Western blot and ELISA tests for HIV: What to expect

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   Jun 12, 2018 · The Western blot and ELISA tests are two blood antibody tests that may be used to detect HIV. In the past, the Western blot test was used to confirm the results of an ELISA test. However,...

7. www.hiv.uw.edu › go › screening-diagnosisCore Concepts - HIV Diagnostic Testing - Screening and ...

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   Oct 7, 2023 · HIV-1 Western Blot Laboratory Tests. The HIV-1 Western blot has been largely replaced by more sensitive and specific HIV diagnostic tests. When used, the HIV-1 Western blot can detect human antibodies that react to HIV-1 proteins that originate from three HIV-1 gene regions: env (gp41, gp120/160), pol (p31, p51, p66), and gag (p15, p17, p24, p55) .

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9. www.cdc.gov › mmwr › previewInterpretation and Use of the Western Blot Assay for ...

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   The Western blot assay is a method in which individual proteins of an HIV-1 lysate are separated according to size by polyacrylamide gel electrophoresis. The viral proteins are then transferred onto nitrocellulose paper and reacted with the patient's serum.