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   Is Western blot test accurate and reliable to detect HIV?

   • This very sensitive test was the first one widely used to check for HIV. The Western blot test was previously used to confirm the result of the ELISA, but it is no longer recommended, as other tests are now more reliable and enable a faster diagnosis.

   Western Blot Test | Stanford Health Care

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   Why is Western blot better than ELISA?

   • •Western Blotting utilizes not only antigens, but also antisera as a diagnostic tool. Antisera is widely used in the test for HIV presence. •Compared to ELISA, Western blotting has higher specificity; the higher specificity, the more the method is independent of the specificity of antibodies.

   western blot and ELISA - ResearchGate

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   Is a positive Western blot proof of HIV infection?

   • The Western blot test separates the blood proteins and detects the specific proteins (called HIV antibodies) that indicate an HIV infection. The Western blot is used to confirm a positive ELISA, and the combined tests are 99.9% accurate.

   Western Blot Test | Stanford Health Care

   stanfordhealthcare.org/medical-conditions/sexual-and-reproductive-health/hiv-aids/diagnosis/western-blot-test.html

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2. www.healthline.com › health › hivWestern Blot Test: Uses, Accuracy, and More - Healthline

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   May 3, 2021 · The Western blot test is used to confirm or contest a diagnosis of HIV or Lyme disease after an ELISA antibody test comes back positive or negative. Since the ELISA test sometimes...

   • Author: Corey Whelan

3. stanfordhealthcare.org › western-blot-testWestern Blot Test | Stanford Health Care

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   A Western blot test is typically used to confirm a positive HIV diagnosis. During the test, a small sample of blood is taken and it is used to detect HIV antibodies, not the HIV virus itself. The Western blot test separates the blood proteins and detects the specific proteins (called HIV antibodies) that indicate an HIV infection.

4. www.verywellhealth.com › how-does-a-western-blotHow Does a Western Blot HIV Test Work? - Verywell Health

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   Jan 21, 2023 · Western blot HIV tests usually look for antibodies against the following HIV proteins: Proteins from the HIV envelope: gp41, and gp120/gp160. Proteins from the core of the virus: p17, p24, p55. Enzymes that HIV uses in the process of infection: p31, p51, p66.

5. www.medicalnewstoday.com › articles › 316230Western blot and ELISA tests for HIV: What to expect

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   Jun 12, 2018 · What are the Western blot and ELISA tests for HIV? Explaining the tests. Preparation. Understanding results. Alternative tests. The Western blot and ELISA tests are two blood antibody tests that...

6. www.cdc.gov › mmwr › previewInterpretation and Use of the Western Blot Assay for ...

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   Enzyme immunoassay (EIA) is the most widely used serologic test for detecting antibody to HIV-1. Serum samples that are repeatedly reactive in the EIA for HIV-1 antibody are then retested with a supplemental and more specific test, the most common of which is the Western blot (1-3).

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8. www.hiv.uw.edu › go › screening-diagnosisCore Concepts - HIV Diagnostic Testing - Screening and ...

   www.hiv.uw.edu › go › screening-diagnosis

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   Oct 7, 2023 · HIV-1 Western Blot Laboratory Tests. The HIV-1 Western blot has been largely replaced by more sensitive and specific HIV diagnostic tests. When used, the HIV-1 Western blot can detect human antibodies that react to HIV-1 proteins that originate from three HIV-1 gene regions: env (gp41, gp120/160), pol (p31, p51, p66), and gag (p15, p17, p24, p55) .