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- The Western blot test is used to confirm or contest a diagnosis of HIV or Lyme disease after an ELISA antibody test comes back positive or negative. Since the ELISA test sometimes produces false positives, a second test is needed to further the diagnosis.
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Jun 12, 2018 · The Western blot and ELISA tests are two blood antibody tests that may be used to detect HIV. In the past, the Western blot test was used to confirm the results of an ELISA test. However,...
However, the Western blot is no longer used, and today the ELISA test is followed by an HIV differentiation assay to confirm HIV infection. The provider may also order an HIV genetic...
May 3, 2021 · The Western blot test is used to confirm or contest a diagnosis of HIV or Lyme disease after an ELISA antibody test comes back positive or negative. Since the ELISA test sometimes...
- Corey Whelan
I know that when an ELISA test indicates a positive result for HIV, a western blot is done to confirm. When I search online for the reason why, all that I see is that western blotting is "more specific" than ELISA. If they both use antibodies, shouldn't these procedures have similarly accurate results?
Nov 2, 2022 · The first advantage of a western blot is that they are highly specific, and are sometimes used to rule out false positives and confirm positive ELISA results in clinical settings. This is due to the initial protein size separation which ensures that non-specific signals from analytes – which would likely be a different size – can be easily ...
Apr 21, 2024 · This confirmatory test is often an immunoblot (western blot) in which HIV peptides from the patient’s blood are identified using an HIV-specific mAb-enzyme conjugate. A positive western blot would confirm an HIV infection and a negative blot would confirm the absence of HIV despite the positive ELISA.
Similar to the western blot, enzyme immunoassays (EIAs) use antibodies to detect the presence of antigens. However, EIAs differ from western blots in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
