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  2. breeding with fresh semen of good quality is planned or when there are no restrictions regarding the use of the stud. Vaginal speculum examinations may save the breeder the cost of progesterone assays; however, for critical breedings (compromised semen, chilled, or frozen semen) and aberrant heat cycles, this method lacks precision. 3

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    • What Hormones Play A Role in ovulation?
    • Physical and Behavioral Changes with LH Surges
    • Pinpointing The LH Surge
    • Length of A Cycle in Relation to Fertile Period
    • Best Practices For Timing Ovulation
    • Semen Quality and Ovulation Timing
    • LH Surge in Relation to C-Sections

    The first hormone of significance is estrogen. Estrogen prepares the reproductive tract for breeding. It causes the vagina to thicken and diapedesis of red blood cells to occur. This is the part of the estrous period that is highly variable bitch to bitch — it may be as short as 1 day or as long as 21 days or even longer. After the estrogen period,...

    Prior to the LH surge, the bitch will have considerable edema, or swelling, and dark red bloody discharge. As estrogen drops and progesterone begins to rise at the start of the LH surge, the edema will go out of the vulva and vagina. You may observe softening of the skin around the vulva and a change in the discharge from a dark bloody red to a mor...

    When breeding highly fertile males or where semen count and quality is not an issue, progesterone blood levels and vaginoscopy can be successfully used to estimate when the LH surge occurred. Levels are typically in the baseline 20 nanograms per milliliter (although levels can be 40 nanograms per milliliter or higher) during the optimum fertile per...

    Typically, bitches are most fertile the last three to four days before they go out of heat. Bitches often follow a similar pattern each heat. So keeping records on every heat even when not breeding will help you be ready for the heat you do breed. Length of estrus is used as a generally guideline to know when to start progesterone levels. Testing f...

    Review the bitches’ heat cycle data with your veterinarian to plan when progesterone levels should start. Share with your veterinarian the behavioral changes you are seeing. Be sure to check progesterone levels until ovulation confirmed. AKC is a participant in affiliate advertising programs designed to provide a means for sites to earn advertising...

    As a general, rule stud dogs should produce 10 million sperm per pound of body weight and have more than 75 percent progressive forward motility with less than 20 percent miscellaneous morphologic defects. Since “normal” dog semen lives another 3-to-7 days in the bitch, the better the semen quality, the more leeway there is in ovulation timing. AKC...

    Bitches whelp 65 days (plus or minus one) from the LH surge regardless of the day bred in the majority of breedings. Therefore, the more accurately you pinpoint the LH surge the more accurately you can narrow down the whelping date. Large litters may come one to two days early and litters of one or two puppies may go one to two days late. AKC is a ...

  3. Feb 17, 2023 · Breeding using fresh chilled or surgically inseminated frozen semen should take place later, when the progesterone level is 20s ng/ml. Of course, if this is the case, you are already working...

  4. Breeders typically have progesterone testing per-formed every two to three days starting five to seven days into the heat cycle. The cost of progesterone testing varies according to individual veterinary clinics and geographical location but averages around $100 to $120 per test. Four to seven progesterone tests are common per breeding cycle.

    • When to start? It is recommended to have the first consultation around six days after the beginning of the heat cycle. Sound too early? Well, always better sooner than later.
    • Very often I had clients call me to say: “Doctor, my bitch is at [number] in progesterone. What should I do?” My typical answer: “[Number] what?” Wonder why?
    • I have been asked to determine when the bitch would ovulate based on a single test. Most of the time that is not possible. (I told you I had bitches that ovulated six days after the beginning of estrus, but I also have had some that ovulated 30 days after!)
    • “Do I need to come every single day until ovulation occurs?” No, this might be necessary when doing LH assays (another hormone secreted by the brain, which peaks two to three days before ovulation).
  5. Sep 18, 2022 · When measuring the progesterone levels in female dogs, breeders should pay attention to ovulation timing. Progesterone level rise and fall are time limited which means that breeders have to choose the right time to breed their female dog. That time limit can be as little as 48 hours, depending on the ovulation cycle.

  6. Progesterone testing is used to determine when a female dog ovulates and when to breed. A reproductive hormone, progesterone increases just before ovulation. Ovulation timing, the telltale indicator that a brood bitch is ovulating and thus ready to breed, is the most common thing to go wrong in dog breeding.

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