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  1. Apr 17, 2023 · There are two forms E. histolytica can take: the cyst form, which can survive in the environment for a prolonged period, and the trophozoite stage, which is the active and invasive form. After the ingestion of the cyst form, trophozoites can be formed, which can invade and penetrate intestinal mucosa destroying epithelial cells and inflammatory ...

  2. Jul 14, 2014 · There are several factors affecting the detection of Entamoeba spp by microscopy which are lack of adequate training in microscopy, delay in delivery of the sample to the laboratory leading to degradation/death of active trophozoite forms, difficulty in differentiation of cyst with degenerated polymorphonuclear cells particularly the mature ...

    • Subhash Chandra Parija, Jharna Mandal, Dinoop Korol Ponnambath
    • 2014
    • Causal Agents
    • Life Cycle
    • Geographic Distribution

    Several protozoan species in the genus Entamoeba colonize humans, but not all of them are associated with disease. Entamoeba histolytica is well recognized as a pathogenic ameba, associated with intestinal and extraintestinal infections. Other morphologically-identical Entamoeba spp., including E. dispar, E. moshkovskii, and E. bangladeshi, are gen...

    Cysts and trophozoites are passed in feces . Cysts are typically found in formed stool, whereas trophozoites are typically found in diarrheal stool. Infection with Entamoeba histolytica (and E.dispar) occurs via ingestion of mature cysts from fecally contaminated food, water, or hands. Exposure to infectious cysts and trophozoites in fecal matter d...

    Pathogenic Entamoeba species occur worldwide and are frequently recovered from fresh water contaminated with human feces. The majority of amebiasis cases occur in developing countries. In industrialized countries, risk groups include men who have sex with men, travelers, recent immigrants, immunocompromised persons, and institutionalized population...

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  4. New approaches to the identification of E. histolytica are based on detection of E. histolytica -specific antigen and DNA in stool and other clinical samples.

    • R. Fotedar, D. Stark, N. Beebe, D. Marriott, J. T. Ellis, J. Harkness
    • 10.1128/CMR.00004-07
    • 2007
    • Clin Microbiol Rev. 2007 Jul; 20(3): 511-532.
  5. Jun 3, 2020 · The life cycle of Entamoeba histolytica is relatively simple, consisting of two stages: the dormant cyst and the vegetative trophozoite ( FIGURE 1 ). Transmission of amoebiasis begins with the ingestion of mature quadrinucleated E. histolytica cysts found in fecal-contaminated food or water ( 40, 133 ).

    • Eileen Uribe-Querol, Carlos Rosales
    • 2020
  6. Nov 12, 2020 · Fact 2: Trophozoites can initiate infection in rodent models. A robust way to culture trophozoites has been developed in the laboratory setting [ 13] and has been the primary resource to perform in vivo infection. Trophozoites are surgically delivered into the target organ within the animal models.

  7. Jan 28, 2021 · E. histolytica is morphologically identical to the noninfectious Entamoeba dispar which lacks key cysteine proteinases that are important for pathogenesis. E. histolytica exists as an infectious cyst with four nuclei. Upon ingestion, it excysts in the terminal ileum to give rise to disease-causing trophozoites with pseudopodia.

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