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      • Biuret's solution is a blue liquid that contains copper sulfate (CuSO 4) and sodium hydroxide (NaOH). Copper sulfate actively binds to the peptide bonds in proteins, forming a structure that transmits a violet color in an alkaline environment. The sodium hydroxide provides the alkaline environment.
      theory.labster.com › biuret

      Biuret's test - Labster

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  3. microbenotes.com › biuret-Biuret Test for Protein: Principle, Procedure, Results, Uses

    May 22, 2024 · Requirements for Biuret Test Biuret Reagent . Alkaline copper sulfate (CuSO 4) solution is used as the Biuret reagent. It is blue in color due to the color of CuSO 4. Components of Biuret Reagent. Copper sulfate (CuSO 4) solution; Sodium potassium tartrate ; Sodium hydroxide (NaOH) or potassium hydroxide (KOH) solution; Distilled water

  4. microbeonline.com › biuret-test-principleBiuret Test: Principle, Procedure, and Uses • Microbe Online

    May 10, 2023 · The test requires the use of a biuret reagent. This reagent is a solution that consists of hydrated copper (II) sulfate, sodium hydroxide, and potassium sodium tartrate. The use of copper (II) ions present in the biuret reagent results in the formation of purple coloration if peptides are present.

  5. biocheminsider.com › biuret-testBiuret Test: Principle, Reagent, Procedure &Result ...

    Apr 9, 2021 · Objective Of Biuret Test. To detect the protein in the given solution. To analyze the presence of the peptide bond in a given analyte. Biuret Reagent. The Biuret reagent is a solution composed of sodium hydroxide (NaOH) or potassium hydroxide (KOH), hydrated copper (II) sulfate, and potassium sodium tartrate.

  6. laboratoryinfo.com › biuret-testWhat does Biuret test for? Its Principle, Mechanism and Uses

    Mar 7, 2022 · Performing biuret test using copper sulphate and sodium hydroxide solutions. Food samples are added with sodium hydroxide solution (1 cm3) and copper (II) sulphate solution (1%). Follow the steps mentioned above but this time with de-ionized water to create a negative control. The other one is for positive control using albumin or egg white.

    • what are the uses of sodium hydroxide in the biuret test method1
    • what are the uses of sodium hydroxide in the biuret test method2
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  7. biochemden.com › biuret-test-for-proteinBiuret Test for Protein- Definition, Principle, Procedure ...

    Jan 6, 2024 · The Biuret Test for Protein is based on the principle that copper ions in the Biuret reagent react with peptide bonds to form a violet-coloured complex. The Biuret reagent contains sodium hydroxide, copper sulphate, and potassium sodium tartrate.

  8. en.wikipedia.org › wiki › Biuret_testBiuret test - Wikipedia

    Biuret reagent. The Biuret reagent is made of sodium hydroxide (NaOH) and hydrated copper(II) sulfate, together with potassium sodium tartrate, the latter of which is added to chelate and thus stabilize the cupric ions. The reaction of the cupric ions with the nitrogen atoms involved in peptide bonds leads to the displacement of the peptide ...

  9. www.biologyonline.com › dictionary › biuret-testBiuret test - Definition and Examples - Biology Online Dictionary

    Mar 3, 2023 · Biology definition: A Biuret test is used for detecting compounds with peptide bonds. A biuret reagent may be used to test the aqueous sample. Thus, the biuret test is used to detect proteins. That is because proteins are made up of polypeptides, which in turn, are made of amino acids joined by peptide bonds.

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