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  2. May 3, 2021 · The Western blot test is used to confirm or contest a diagnosis of HIV or Lyme disease after an ELISA antibody test comes back positive or negative. Since the ELISA test...

    • Corey Whelan
  3. The Western blot test separates the blood proteins and detects the specific proteins (called HIV antibodies) that indicate an HIV infection. The Western blot is used to confirm a positive ELISA, and the combined tests are 99.9% accurate.

  4. Jan 21, 2023 · Western blot HIV tests usually look for antibodies against the following HIV proteins: Proteins from the HIV envelope: gp41, and gp120/gp160. Proteins from the core of the virus: p17, p24, p55. Enzymes that HIV uses in the process of infection: p31, p51, p66.

  5. Jun 12, 2018 · The Western blot and ELISA tests are two blood antibody tests that may be used to detect HIV. In the past, the Western blot test was used to confirm the results of an ELISA test. However,...

  6. Oct 7, 2023 · HIV-1 Western Blot Laboratory Tests. The HIV-1 Western blot has been largely replaced by more sensitive and specific HIV diagnostic tests. When used, the HIV-1 Western blot can detect human antibodies that react to HIV-1 proteins that originate from three HIV-1 gene regions: env (gp41, gp120/160), pol (p31, p51, p66), and gag (p15, p17, p24, p55) .

  7. Enzyme immunoassay (EIA) is the most widely used serologic test for detecting antibody to HIV-1. Serum samples that are repeatedly reactive in the EIA for HIV-1 antibody are then retested with a supplemental and more specific test, the most common of which is the Western blot (1-3).

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