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Limulus amebocyte lysate ( LAL) is an aqueous extract of motile blood cells ( amebocytes) from the Atlantic horseshoe crab Limulus polyphemus. LAL reacts with bacterial endotoxins such as lipopolysaccharides (LPS), which are components of the bacterial capsule, the outermost membrane of cell envelope of gram-negative bacteria.
Jan 8, 2019 · Limulus amebocyte lysate (LAL) is an aqueous extract of blood cells (amoebocytes) from the horseshoe crab, Limulus polyphemus. LAL reagent reacts with bacterial endotoxin and lipopolysaccharide (LPS), which is a membrane constituent of Gram-negative bacteria.
Dec 3, 2020 · When those cells meet invading bacteria, they clot around it and protect the rest of the horseshoe crab's body from toxins. Scientists used these clever blood cells to develop a test called Limulus Amebocyte Lysate, or LAL, which checks new vaccines for contamination.
Currently the in vitro limulus amebocyte lysate (LAL) test is the assay of choice for the determination of endotoxin contamination. The LAL assay exists in three formats, namely chromogenic, turbidity, or gel-clot assessments.
Mar 15, 2007 · A hemocyte lysate from horseshoe crab (Limulus) produced a gel, when exposed to Gram-negative bacterial endotoxins, lipopolysaccharides (LPS). This gelation reaction of the lysate, so-called Limulus test, has been widely employed as a simple and very sensitive assay method for endotoxins.
May 11, 2021 · The Limulus amebocyte lysate (LAL) obtained from horseshoe crab blood cells functions as a surprisingly sophisticated sensing system that allows for the extremely sensitive detection of bacterial and fungal cell-wall components.
Abstract This year celebrates the 30th anniversary of the licensing of Limulus amebocyte lysate (LAL) by the US Food and Drug Administration (FDA) as a test for the presence of endotoxin in biologicals, pharmaceutical drugs, and medical devices. LAL is currently recognized by several major pharmacopoeias and is used worldwide.
Jan 1, 2009 · This year celebrates the 30th anniversary of the licensing of Limulus amebocyte lysate (LAL) by the US Food and Drug Administration (FDA) as a test for the presence of endotoxin in biologicals, pharmaceutical drugs, and medical devices.
Test Overview. Test Methodology. Kinetic Assay. Test Usage. Detection and quantitation of gram-negative endotoxin in aqueous solutions used in patient management. For investigational use only. Reference Range * < 0.05 EU/ml (Negative). Interpretive report provided. * Reference ranges may change over time.
For more than 30 years, FDA has accepted the use of a Limulus Amoebocyte Lysate (LAL) test for endotoxins in lieu of the rabbit pyrogens test.
