Search results
STORAGE OF E. COLI FROZEN STOCKS IN GLYCEROL. For long-term storage, E. coli can be mixed with glycerol (final concentration of 30%) and stored at −80°C for an indefinite amount of time. These frozen stocks will be the source of all future experiments in which this particular strain will be used.
Some bacterial strains can be stored for up to 1 year at 4°C in agar stab cultures, which are especially useful for transporting samples to other research facilities. Stab cultures are prepared by first sterilizing strain-compatible agar (e.g., lysogeny broth [LB] agar for E. coli) and then transferring the warm liquid agar to screw-cap vials ...
People also ask
Can E coli strains be stored at 80°C?
How long can E coli be stored?
How long can E coli survive at 4°C?
Some say one should store the pellet after centrifugation at +4°C, some suggest -20°C, and some -80°C. So - what is your experience? Is there an optimal way? Does it matter at all?
Mar 8, 2021 · I know that a -80°C freezer is the recommended means of long-term cell-line storage, and that cells will typically not last long in a -20°C freezer. But how long will a typical bacterial strain (e.g., E. coli DH5-alpha) actually stay good in a -80?
Feb 28, 2012 · If all you have at the moment is a -20°C and mostly what you want to store is E. coli harboring plasmids, I'd recommend preparing plasmid DNA and storing that at -20°C. The DNA will stay stable in the medium term, and you can re-transform into E. coli once you've got your -80°C freezer up and running.
- I think that cells get damaged at -20°C not because they are stored for a long period of time, but because they undergo cycles of thawing and freez...
- Gergana covered the "why" part of your question. +1 If all you have at the moment is a -20°C and mostly what you want to store is E. coli harbori...
- The answers already posted about glycerol being a cryoprotectant and ice crystals are correct. The other thing to consider is that enzymes still re...
If you are going to store the bacteria in a special -80°C freezer, you should first snap-freeze the bacterial stock by dropping it in a container of liquid nitrogen. If you are storing the bacteria in a regular -20°C freezer, the bacterial stock can be placed there with no further treatment.
WORKING WITH E. COLI CELLS MAKING GLYCEROL STOCKS OF E. COLI STRAINS Glycerol stocks are the best way to store bacterial strains at -80 °C. Glycerol is a cryoprotectant which will help the culture to survive under frozen conditions. These frozen cultures are stored at -80 °C and are used for "plating out" colonies. Everyone in the lab
