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Modern technologies have enabled labs to improve how they go about pipetting small volumes with precision and accuracy. Today, you can pipette small volumes (usually between 0.2 and 20 μL) with an accurate, tested, and proven technique.
May 7, 2017 · Several factors explain this trend: high-value samples, the ability to detect changes accurately in low volumes, and the continuing replacement of radiolabeling with optical (particularly fluorescence) techniques that enable multiplexed assays.
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Can You pipette a small volume at low temperature?
- Choose The Right Pipette and Pipette Tips
- Check That Your Pipettes Are Calibrated
- Dilute Highly Concentrated Stock Solutions
- Avoid Foaming and Bubbles
- Thoroughly Mix The Contents of The Well
- Prevent Evaporation by Covering Your Plate
Volumetric errors are amplified at such low volumes. To mitigate these errors, be sure to select the proper pipette that delivers volumes that match your protocol. As a rule of thumb, select the smallest pipette capable of handling the volume you need. Pipetting accuracy decreases the further your pipetted volume is to the maximum volume of the pip...
Be sure the pipettes you’re using are also calibrated appropriately. You can do a quick check using distilled water and a microbalance. Since the density of water is 1g/mL, each microliter of distilled water at room temperature should weigh 1mg.
Working with highly concentrated stock solutions requires working with even lower volumes. If your wells can handle additional volume, prepare intermediate solutions. For example, work with 2X or 5X stock solutions instead of 10X if you can.
Whether you’re weighing the benefits of popping bubbles with the pipette tip (without introducing more bubbles!), or you’re resigned to starting over, there are some preemptive measures you can take to avoid this problem in the first place. Try reverse pipetting. Although this requires a little extra volume, bubbles and foaming are greatly reduced....
When handling small volumes, mixing is particularly important as handling mistakes are much more obvious. When mixing by pipetting, be sure to set the volume on your pipette equal to at least half the volume in the well you want to mix. While this can be done manually, you can save a lot of finger fatigue by learning how to set mixing programs on a...
Evaporation is always an issue when handling liquids, and the effects are magnified when working with small volumes. Use plastic plate covers after you are done or if you need to take a break. The plate remains covered until you are ready to use it. Alternatively, you can use parafilm or sealing tape to help prevent evaporation. Although pipetting ...
- Patricia Piatti
- Choose the right pipette and pipette tips. Volumetric errors are amplified at such low volumes. To mitigate these errors, be sure to select the proper pipette that delivers volumes that match your protocol.
- Check that your pipettes are calibrated. Be sure the pipettes you’re using are also calibrated appropriately. You can do a quick check using distilled water and a microbalance.
- Dilute highly concentrated stock solutions. Working with highly concentrated stock solutions requires working with even lower volumes. If your wells can handle additional volume, prepare intermediate solutions.
- Avoid foaming and bubbles. Whether you’re weighing the benefits of popping bubbles with the pipette tip (without introducing more bubbles!) , or you’re resigned to starting over, there are some preemptive measures you can take to avoid this problem in the first place.
Aug 2, 2021 · Knowing how to measure volumes of liquids accurately using pipets, is an important skill that can be applied to experiments in tissue culture, molecular and cell biology and immunology labs. Plastic pipets (“squeezers”), calibrated to 1 or 2 mL are also used to transfer small volumes of liquids.
- Tobili Y. Sam-Yellowe
- 2021
Sir Gregory P. Winter delivered his Nobel Lecture on 8 December 2018 at the Aula Magna, Stockholm University. He was introduced by Professor Sara Snogerup Linse, member of the Nobel Committee for Chemistry.
Oct 3, 2018 · Winter developed and improved this technology, and invented ways to use it to evolve antibodies adapted for use as human therapeutics. Today, antibodies evolved using this method can neutralize...
