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  2. These tips on how to pipette will instantly improve your pipetting technique. Download our free poster on how to correctly use a pipette.

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  1. Apr 29, 2015 · Incompatible pipette tips can reduce accuracy and precision so it is important that you choose a high-quality pipette tip suited to your pipette. Low retention tips are recommended for high accuracy. Pre-wetting – It is important that you pre-wet the pipette tip for a couple of reasons. Firstly, it allows you to flush out residual substances ...

  2. That's is why some companies sell something call low retention pipette tips to avoid the issue. I don't think tips need to be pre-wet. They are not designed to be used like that. But, if you pre-wet your tips, pre-wet it every time to be consistent. You can use a fine balance and water to test your pipette accuracy. 1000ul is exactly 1g.

  3. Learn how to correctly use a pipette and improve your results instantly. This article explains the following ten tips in detail: Ensure temperature equilibrium: Make sure the pipette, tips and liquids are at room temperature (if experiment allows) Maintain consistent pipette angle: Hold the pipette at a consistent angle not exceeding 20 degrees.

  4. Sep 22, 2020 · 1. Pre-Wet the Pipette Tip. Before aspirating any sample for your experiment or delivery, you should aspirate and dispense the sample liquid at least three times to pre-wet the tip. If the tip is not pre-wetted, significant sample loss may be caused by the evaporation of liquid within the tip. By pre-wetting, the humidity of the tip increases ...

  5. Reproducibility can be increased in experiments further by using an electronic pipette and multi-dispensing mode. In this informative one-hour webinar, we discuss the importance of best practices when pipetting for qPCR, including: Keeping pipettes well-maintained for qPCR. Recognizing limiting factors affecting your results.

  6. Feb 16, 2023 · Before setting up your serial dilution, you should perform a pre-wet of your pipette tips. Do this by aspirating and dispensing the full volume of diluent two to three times. This procedure not only equilibrates temperature differences between the liquid and the tip, but also humidifies the dead air space inside the pipette and tip.

  7. Nov 4, 2020 · If you’re pipetting viscous liquids, make sure to do it at a slower speed and work in reverse pipette mode. When pipetting volatile liquids, do it quickly and use the reverse pipette mode. 3. Pre-wet the tip: To reduce variations in volume delivery, pre-wet your tip at least three times. By doing so, you can help create a temperature ...

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  2. Free shipping on qualified orders. Free, easy returns on millions of items. Find deals and low prices on filter tips pipette at Amazon.com

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