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CCL-25. ™. The WISH human cell line contains HeLa marker chromosomes and were derived via HeLa contamination. This cell line was deposited by L Hayflick. Product category. Human cells. Organism. Homo sapiens, human. Morphology.
Mar 11, 2013 · HeLa was the first human cell line established in culture (Gey et al. 1952) and has since become the most widely used human cell line in biological research.Its application as a model organism has contributed to the characterization of important biological processes and more than 70,000 publications.
Omics: Virome analysis using proteomics. Anecdotal: The HeLa cell line which was established in February 1951 is the oldest human immortal cell line. Anecdotal: The fascinating story of the HeLa cell line and of Henrietta Lacks from whom these cells originate are described in the book of Rebecca Skloot (CelloPub= CLPUB00377 ).
Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.
Aug 7, 2013 · The HeLa cell line was established in 1951 from cervical cancer cells taken from a patient, Henrietta Lacks. ... HeLa cell culture. HeLa cell cultures (HeLa ATCC, CCL-2 (laboratory stock); HeLa S3 ...
Mar 22, 2016 · The growth profile of individual HeLa cells varied significantly, and the majority of cells were mortal, in contrast to the general belief that HeLa cells are a cell line composed of immortal cells. Furthermore, only a small number of cells (3.2–6.1%) retained immortal growth ability, and gave rise to the remaining cell population.
Sep 12, 2016 · HeLa human epithelial cell lines were purchased from ATCC #CCL-2 (Manassas, Virginia, USA) and cultured using the standard ATCC protocol. HeLa cells were cultured overnight on the apical compartment of a 12-well Transwell ® apparatus (Corning Inc.; Corning, New York, USA). For our proof-of-principle experiments, three different Transwell ...
