Robert Tinkler
Slime
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Episode Guide
- 1. The Show Must Go On Jan 5, 2012
- In an online contest to become real rock stars, four teens form a virtual band called Mudpit.
- 2. Avatarts Jan 12, 2012
- Mudpit searches for a replacement when Geneva joins a girl group called the Avatarts .
- 3. Travelin Band Jan 19, 2012
- Mudpit members bond as they pull an all-nighter for a challenge.
MudPIT is a non-gel technique for separating and identifying individual components of complex protein and peptide mixtures.
Mudpit follows the adventures of four teens who form the eponymous band, Mudpit, as they try to earn a recording contract through competing in the Polish massively multiplayer online role-playing game Muzika.
Multidimensional Protein Identification Technology (MudPIT) eliminates gel separations. Instead, biochemical fractions containing many proteins are directly proteolyzed and the enormous number of peptides generated, are separated by 2-dimensional liquid chromatography before entering the mass spectrometer.
MUDPIT (multidimensional protein identification technology) is the method of choice for complex protein sample analysis in which more elaborate separation techniques are needed.
MudPIT combines both a cation exchange pre-fractionation and RP HPLC separation (1) of tryptic peptides to analyze an entire proteome of a cell or tissue type protein extract.
Characterization of protein mixtures using HPLC/MS is also called shotgun proteomics and MuDPIT (Multi-Dimensional Protein Identification Technology). A peptide mixture that results from digestion of a protein mixture is fractionated by one or two steps of liquid chromatography.
We describe a largely unbiased method for rapid and large-scale proteome analysis by multidimensional liquid chromatography, tandem mass spectrometry, and database searching by the SEQUEST...
