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A Gram stain is a laboratory test that checks for bacteria at the site of a suspected infection or in certain bodily fluids. A medical laboratory scientist processes the Gram stain, which gives relatively quick results, so healthcare providers can know if bacteria are present, and, if so, the general type (s).
Aug 14, 2023 · The Gram staining is one of the most crucial staining techniques in microbiology. It gets its name from the Danish bacteriologist Hans Christian Gram, who first introduced it in 1882, mainly to identify organisms causing pneumonia.[1] Often, the first test performed, gram staining, involves the use of crystal violet or methylene blue as the primary color.[2] The term for organisms that retain ...
- Nishant Tripathi, Amit Sapra
- 2023/08/14
Gram stain of Candida albicans from a vaginal swab. The small oval chlamydospores are 2–4 µm in diameter. Gram staining is a bacteriological laboratory technique used to differentiate bacterial species into two large groups (gram-positive and gram-negative) based on the physical properties of their cell walls.
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Table 1: Summary of some common differential stains used in microbiology. The Gram stain uses the following dyes/reagents: crystal violet, Gram's iodine, ethanol, and safranin. The Gram stain distinguishes cells by cell wall type (Gram-positive or Gram negative). Gram-positive cells stain purple/violet.
Aug 10, 2022 · Procedure of Gram Staining. Take a clean, grease free slide. Prepare the smear of suspension on the clean slide with a loopful of sample. Crystal Violet was poured and kept for about 30 seconds to 1 minutes and rinse with water. Flood the gram’s iodine for 1 minute and wash with water. Then ,wash with 95% alcohol or acetone for about 10-20 ...
Feb 4, 2022 · Flood the Gram’s Iodine solution over the smear. Leave the iodine solution for 30 – 60 seconds and pour off the excess iodine and rinse with gentle running water. Shake off the excess water over the smear. Decolorize the smear by passing the decolorizing solution till the solution runs down in clear form.
Apr 13, 2016 · Gram Staining. Gram staining is a diagnostic test that gives an early indication of potential bacteria through visualization of the bacteria. The Gram stain helps to differentiate the organism, whether it is gram-positive or gram-negative. 50 Gram-positive bacteria appear purple in color and gram-negative bacteria appear pink. In addition, the ...
