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The Klenow fragment is a large protein fragment produced when DNA polymerase I from E. coli is enzymatically cleaved by the protease subtilisin.
This undesirable enzymatic activity can be simply removed from the holoenzyme to leave a useful molecule called the Klenow fragment, widely used in molecular biology. In fact, the Klenow fragment was used during the first protocols of polymerase chain reaction (PCR) amplification until Thermus aquaticus , the source of a heat-tolerant Taq ...
In the bacterial thermostable DNA polymerases, a Klenow fragment (Klen-Taq) or a Stoffel fragment can be generated by deleting the exonuclease domain in the course of protein design, analogous to the DNA polymerase from E. coli, which results in a higher product concentration.
A Klenow fragment is a protein fragment derived from DNA polymerase I through proteolysis, often used in recombinant DNA technology for its DNA polymerase activity. AI generated definition based on: Enzymology Primer for Recombinant DNA Technology, 1996
Following its discovery by Klenow and Henningsen (1970), the large fragment has been called the “Klenow fragment.” In order to polymerize the addition of nucleotides to the 3′‐OH end of DNA, the Klenow fragment requires a single‐stranded DNA template and a DNA or RNA primer with a 3′‐OH terminus.
DNA Polymerase I, Large (Klenow) Fragment is a proteolytic product of E. coli DNA Polymerase I which retains polymerization and 3'→ 5' exonuclease activity, but has lost 5'→ 3' exonuclease activity (1). Klenow retains the polymerization fidelity of the holoenzyme without degrading 5' termini.
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What is a Klenow fragment?
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What are functional domains in Klenow fragment & DNA polymerase I?
Klenow Fragment is a mesophilic DNA polymerase derived from the E. coli Polymerase I DNA-dependent repair enzyme. The enzyme exhibits DNA synthesis and proofreading (3ʹ→5ʹ) nuclease activities and, in the absence of the holoenzyme’s (5ʹ→3ʹ) nuclease domain, displays a moderate strand displacement activity during DNA synthesis.
