Search results
People also ask
What are the results of the biuret test?
Why is a biuret test called a peptide test?
Why is the biuret reaction used to assess the concentration of proteins?
Apr 16, 2023 · Requirements for Biuret Test Biuret Reagent . Alkaline copper sulfate (CuSO 4) solution is used as the Biuret reagent. It is blue in color due to the color of CuSO 4. Components of Biuret Reagent. Copper sulfate (CuSO 4) solution; Sodium potassium tartrate ; Sodium hydroxide (NaOH) or potassium hydroxide (KOH) solution; Distilled water
May 10, 2023 · The test requires the use of a biuret reagent. This reagent is a solution that consists of hydrated copper (II) sulfate, sodium hydroxide, and potassium sodium tartrate. The use of copper (II) ions present in the biuret reagent results in the formation of purple coloration if peptides are present.
Apr 9, 2021 · Objective Of Biuret Test. To detect the protein in the given solution. To analyze the presence of the peptide bond in a given analyte. Biuret Reagent. The Biuret reagent is a solution composed of sodium hydroxide (NaOH) or potassium hydroxide (KOH), hydrated copper (II) sulfate, and potassium sodium tartrate.
Mar 7, 2022 · Performing biuret test using copper sulphate and sodium hydroxide solutions. Food samples are added with sodium hydroxide solution (1 cm3) and copper (II) sulphate solution (1%). Follow the steps mentioned above but this time with de-ionized water to create a negative control. The other one is for positive control using albumin or egg white.
Biuret reagent. The Biuret reagent is made of sodium hydroxide (NaOH) and hydrated copper(II) sulfate, together with potassium sodium tartrate, the latter of which is added to chelate and thus stabilize the cupric ions. The reaction of the cupric ions with the nitrogen atoms involved in peptide bonds leads to the displacement of the peptide ...
Jan 6, 2024 · Principle. The Biuret Test for Protein is based on the principle that copper ions in the Biuret reagent react with peptide bonds to form a violet-coloured complex. The Biuret reagent contains sodium hydroxide, copper sulphate, and potassium sodium tartrate.
A small amount of this aqueous solution must be treated with 1% sodium hydroxide or potassium hydroxide. To this mixture, a few drops of CuSO 4 (aq) must be added. If the solution turns purple upon the addition of copper(II) sulphate, the presence of protein in the analyte is confirmed.
