Search results
Find various methods and kits for isolating and purifying DNA from different sources, such as blood, tissue, plants, and bacteria. Compare protocols for cosmid, genomic, plasmid, and PCR DNA extraction.
Jul 3, 2023 · Basic Protocol 1 describes the extraction of DNA from human blood, tissue, or cultured cells using a column-based kit such as the QIAamp DNA Blood Mini Kit or the DNeasy Blood and Tissue Kit. Though far from an exhaustive list, Table 1 provides information about most of the commonly used DNA extraction kits in research laboratories.
Polymerase chain reaction (PCR) is a robust technique to selectively amplify a specific segment of DNA in vitro . [ 1] PCR is performed on thermocycler and it involves three main steps: (1) denaturation of dsDNA template at 92–95°C, (2) annealing of primers at 50–70°C, and (3) extension of dsDNA molecules at approx. 72°C.
- Nalini Gupta
- 2019
- Plant Genomic DNA Isolation
- Serum-Plasma Genomic DNA Isolation
- Bacterial Genomic DNA Isolation
- GeneratedCaptionsTabForHeroSec
The Wizard® Magnetic 96 DNA Plant System (Cat.# FF3760, FF3761) is designed for manual or automated 96-well purification of DNA from plant leaf and seed tissue. The Wizard® Magnetic 96 DNA Plant System has been validated with corn and tomato leaf as well as with canola and sunflower seeds. The DNA purified from these samples can be used in PCR and ...
For high quality, purified cell-free DNA from plasma samples, we offer the Maxwell® RSC ccfDNA Plasma Kit (Cat.# AS1480). Utilizing the simple three-step protocol, the Maxwell® RSC Instrument can process 1 to 16 samples, and the Maxwell® RSC 48 Instrument can process 1 to 48 samples. Simply add 0.2–1.0ml of plasma to the prepared cartridges and sel...
If you need to make quick decisions about potential food contamination and spoilage, the Maxwell® RSC PureFood Pathogen Kit (Cat.# AS1660) offers a simple automated protocol with minimal hands-on steps. The kit effectively eliminates laborious sample preprocessing steps such as enzymatic pretreatment, as it works with inhibiting sample types and al...
Learn about the basics of DNA extraction, plasmid preparation and nucleic acid quantification from Promega. Compare different methods and products for genomic, plasmid and fragment/PCR product purification.
Oct 23, 2018 · Learn how to isolate and purify genomic DNA from cells using the Monarch gDNA Purification Kit. Follow the detailed steps for sample lysis, binding, wash and elution, and download the quick protocol card.
Centrifuge the sample at 4°C for 2 minutes at 16,000 × g. Carefully remove the supernatant. Repeat Step 3 once. Remove as much of the remaining ethanol as possible. Dry the cDNA pellet in a Thermo Scientific™ SpeedVac ™ concentrator for 2 minutes or at room temperature for 5–10 minutes. Resuspend the cDNA pellet in 300 μL of TEN buffer ...
People also ask
What are the different types of DNA extraction methods?
Can automated DNA extraction machines help with high-throughput DNA isolation?
How long does it take to extract DNA from tissue?
What is a DNA purification guide?
Jul 11, 2023 · 7.3: DNA Extraction is shared under a license and was authored, remixed, and/or curated by LibreTexts. DNA is the instruction manual for living things. By far, the greatest amount of DNA is located in the nucleus of eukaryotic cells and described as a double-helix. The double-stranded genetic ….
