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  1. E. coli stains gram-negative because its cell wall is composed of a thin peptidoglycan layer and an outer membrane. During the staining process, E. coli picks up the color of the counterstain safranin and stains pink.

    • EPEC

      Escherichia coli (/ ˌ ɛ ʃ ə ˈ r ɪ k i ə ˈ k oʊ l aɪ /...

    • Gram-negative

      Gram-negative bacteria are bacteria that unlike...

    • Enterobacteriaceae

      Enterobacteriaceae is a large family of Gram-negative...

  2. Apr 13, 2016 · This paper reviewed core concepts of interpreting bacterial culture results, including timing of cultures, common culture sites, potential for contamination, interpreting the Gram stain, role of rapid diagnostic tests, conventional antibiotic susceptibility testing, and automated testing.

    • Christopher Giuliano, Chandni R Patel, Pramodini B Kale-Pradhan
    • CrossRef
    • 2019
    • P T. 2019 Apr; 44(4): 192-200.
  3. Escherichia coli (commonly abbreviated E. coli) is a Gram-negative, rod-shaped bacterium that is commonly found in the lower intestine of warm-blooded organisms (endotherms). Most E. coli strains are harmless, but some serotypes can cause serious food poisoning in humans.

  4. Jan 1, 2008 · E. coli is Gram-negative and its envelope has three layers: cytoplasmic membrane, peptidoglycan, and outer membrane. The peptidoglycan is rigid determining the rod shape. To a good approximation, the E. coli cell has hemispherical caps and a cylindrical section in between.

    • Galina Reshes, Sharon Vanounou, Itzhak Fishov, Mario Feingold
    • 10.1529/biophysj.107.104398
    • 2008
    • Biophys J. 2008 Jan 1; 94(1): 251-264.
  5. Dec 31, 2022 · Escherichia coli have served as important model organisms for over a century—used to elucidate key aspects of genetics, evolution, molecular biology, and pathogenesis. However, defining which strains actually belong to this species is erratic and unstable due to shifts in the characters and criteria used to distinguish bacterial species.

    • Marta Cobo-Simón, Rowan Hart, Howard Ochman
    • Mol Biol Evol. 2023 Jan; 40(1): msac273.
    • 10.1093/molbev/msac273
    • 2023/01
  6. Tell what the Gram stain tells us about different species of bacteria. Examine Gram-stained cells and interpret whether the cells are Gram-positive or Gram-negative. Identify cell morphology of bacteria. Describe the structure of the cell walls of Gram-positive cells.

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  8. Morphology and Staining of Escherichia Coli: E. coli is Gram-negative straight rod, 1-3 µ x 0.4-0.7 µ, arranged singly or in pairs (Fig. 28.1). It is motile by peritrichous flagellae, though some strains are non-motile. Spores are not formed. Capsules and fimbriae are found in some strains.

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