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- The process of making a smear preparation is an important skill in the microbiology laboratory and is usually the first step in most staining procedures. The quality of the smear will directly affect the quality of the subsequent staining procedure. The smear preparation differs slightly depending on the specimen or culture.
www.cdc.gov › labtraining › docsSmear Preparation - Centers for Disease Control and Prevention
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Spread the drop into a thin, even smear. add one drop of sterile saline or sterile water to the center of the microscope slide. Aseptically pick a small amount of an isolated colony with a loop and gently mix into the drop of sterile saline or water using circular motions. Mix evenly to make a thin smear. Allow the smear to air dry completely.
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May 18, 2021 · Part I: Preparation of a Bacterial Smear . This semester you will be performing two staining procedures: A Simple Stain and a Gram stain. Both of these staining procedures begin with the preparation of a bacterial smear. Materials. Clean microscope slides; Staining trays and newspaper; Water bottle (for rinsing)
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The smear preparation is what causes the cells to adhere to the microscope slide so that they are not washed off during the staining process. Heat needs to be applied in order for this adherence to occur. It is this heat that causes cell death during the staining process.
Using a sterile dropper, gently mix and remove some of the specimen from the tube and place one drop (10μL) on a clean microscope slide with the patient’s identification number/name. Immediately put a coverslip over the sample for examination. A microscopic review of the slide should be performed as soon as possible to confirm the presence ...
May 26, 2021 · Discuss how to prepare a bacterial smear from cultured organisms. Distinguish between Gram-positive and Gram-negative bacteria. Describe the process of the Gram stain procedure. Use microscopy to examine Gram stained cells. Describe select special staining procedures and view examples of these under oil immersion.
