Search results
Jun 3, 2020 · The life cycle of Entamoeba histolytica is relatively simple, consisting of two stages: the dormant cyst and the vegetative trophozoite . Transmission of amoebiasis begins with the ingestion of mature quadrinucleated E. histolytica cysts found in fecal-contaminated food or water ( 40 , 133 ).
- Eileen Uribe-Querol, Carlos Rosales
- 2020
The stage conversion process remains poorly understood despite being central to amoebic biology. In this review, we will highlight recent progress in our understanding of Entamoeba stage conversion including dissecting transcriptome analysis in development, characterization of transcriptional networks, demonstration of epigenetic regulation ...
- Dipak Manna, Gretchen M Ehrenkaufer, Daniela Lozano-Amado, Upinder Singh
- 2020
People also ask
What is the life cycle of Entamoeba histolytica?
Why is Entamoeba invadens a good model for excystation and encystation?
Does Entamoeba histolytica have a trophozoite stage?
May 26, 2016 · Extracts were aliquoted and stored at –70°C until used. Cyst protein extract was prepared from a pool of isolated cysts (about 2 × 10 5) from 10 PCR-positive samples for E. histolytica, as follows: cysts were washed and placed in 500 μl of PBS with protease inhibitors (Complete tablet, Sigma) on ice for 15 min.
- Milka Luna-Nácar, José Navarrete-Perea, Bárbara Moguel, Raúl J. Bobes, Juan P. Laclette, Julio C. Ca...
- 10.1371/journal.pone.0156018
- 2016
- PLoS One. 2016; 11(5): e0156018.
Fact 1: Rodent models do not mimic the entire life cycle of E. histolytica. Entamoeba histolytica is an extracellular enteric eukaryotic parasite. Globally, an average of 50 million cases and 55,000 to 100,000 deaths are due to E. histolytica infection each year, primar-ily impacting the developing world [1,2].
- Carolina Mendoza Cavazos, Laura J. Knoll
- 2020
- Historical Background
- Morphology and Biology
- Epidemiology
- Amebiasis in The Mediterranean Countries
- Pathophysiology
- Clinical Picture
- Ameboma
- Laboratory Diagnosis
- Microscopy
- Culture
Ronald Elsdon Dew made a full comprehensive description of the history of amoebae in a review article published in 1969 . He mentioned that Lösch was the first to report the amoebic trophozoites in the stools of a Russian patient in St. Petersburg suffering from severe dysentery in 1875. Definite association of amoeba with disease was established b...
Entamoeba histolytica occurs in the following forms, the trophozoites, precyst, cyst, metacyst, and metacystic trophozoite. The trophozoite is about 10–60 μm in size with a clear finely granular cytoplasm and a spherical nucleus that shows aggregation of chromatin beads at the nuclear membrane and a centrally located karyosome. The trophozoite is t...
Amebiasis is responsible for around 100,000 deaths/year, mainly in Central and South America, Africa, and India, as well as for a significant rate of morbidity manifested as invasive intestinal or extraintestinal disease . Infection with E. histolytica has been estimated to be as high as 50% in some developing countries as South and Central America...
The Mediterranean countries cover parts of the three old continents Africa, Asia, and Europe with an area of 2,085,292 km2 “(https://en.wikipedia.org/wiki/Mediterranean_Basin).” Its climate is generally moderate; however, it differs according to many factors as topography, plant cover, and rain fall. These differences plus political and financial s...
Amoebiasis is thought to be one of the most commonly known parasitic diseases affecting millions of people worldwide [57, 58]. Many of the cases have been discovered accidentally during a routine stool analysis and unfortunately many of them were given unneeded treatment for ameba. Those cases infected with the organism without symptoms were called...
Infection may be asymptomatic in most of the cases; however, severe fulminating disease may occur after an incubation period of 7–28 days from exposure to infection. Mild symptoms may be in the form of abdominal cramps, diarrhea with passage of 3–8 soft stools/day, or passage of stool with mucus and occasional blood. There may be fatigue, excessive...
It is one of the rare complications of colonic infection with pathogenic E. histolytica. It occurs as a result of deep invasion of the wall of the colon by invasive E. histolytica trophozoites with extensive formation of granulation tissue and the development of tumor-like mass. The most common sites of ameboma are the cecum and ascending colon. It...
Amebiasis is diagnosed in the laboratory by detecting the parasite in specimens or by an immunologic or a molecular technique [79,80,81,82,83]. In case of invasive intestinal amebiasis, the blood picture in 80% of cases may show leukocytosis without eosinophilia, elevation of erythrocytic sedimentation rate (ESR), elevation of serum alkaline phosph...
Identification of haematophagous trophozoites in fresh stool smears is the primary step for the identification of intestinal amebiasis and is an indication of infection [1•]. Because of irregular output of the parasite stages in stool, a single stool analysis is not usually positive. To further improve the sensitivity of the microscopic examination...
Culture is a method of diagnosis, however it is not easy to perform and less sensitive than microscopic examination with a success rate of 50–70% [1•]. Xenic cultivation, first introduced in 1925, is defined as the growth of the parasite in the presence of an undefined flora. This technique is still in use today utilizing the modified Locke egg or ...
- Nadia A. El-Dib
- naeldib@hotmail.com
- 2017
Jan 1, 2014 · The life cycle of E. histolytica has been described by Dobell (1928) and comprises an infective cyst form, metacystic trophozoite, invasive motile feeding trophozoite, precyst and metacyst stages. Infection occurs following ingestion of mature cysts (10–16 μm) which contain up to four nuclei, in faecally contaminated food, water, on hands or ...
Oct 1, 2014 · Amoebiasis is a disease caused by the cosmopolite pa rasitic protozoa E. histolytica; it has been estimated that the 10% of. the world population is infected by this protozoa, which repr esents ...
