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Mar 30, 2015 · Light is an important environmental cue that affects physiology and development of Neurospora crassa. The light-sensing transcription factor (TF) WCC, which consists of the GATA-family TFs WC1 and WC2, is required for light-dependent transcription. SUB1, another GATA-family TF, is not a photoreceptor but has also been implicated in light ...
- Light-Dependent and Circadian Transcription Dynamics In Vivo Recorded With a Destabilized Luciferase Reporter in Neurospora
We show that firefly luciferase is a stable protein when...
- PMC Free Article
Rhythmic nucleosome occupancy at the frq locus. To...
- Light-Dependent and Circadian Transcription Dynamics In Vivo Recorded With a Destabilized Luciferase Reporter in Neurospora
The WC1- population is found in large numbers in spleen and intestine. In addition, this subset is not recognized by a number of monoclonal antibodies (mAbs) specific for TCR families that are well represented in the WC1+ population.
- Niall D. MacHugh, J.K. Mburu, M.J. Carol, C.R. Wyatt, J.A. Orden, W.C. Davis
- 10.1046/j.1365-2567.1997.00350.x
- 1997
- Immunology. 1997 Nov; 92(3): 340-345.
- Annotation of WC1 Genes
- CDNA Evidence For WC1 Distinct Gene Structures
- Domain 1 Is The Most Variable SRCR Domain
- Intracytoplasmic Region Analysis and Classification
- Evidence For Isoform Generation
Here we set out to annotate the bovine genome to confirm the existence of the large number of WC1 genes (10–13 genes) transcript analyses supported for cattle [11, 48]. While diversity in cattle WC1 molecules could result from alternative gene splicing as occurs for another T cell expressed-SRCR gene CD6 , Southern blots indicated up to 50 WC1 gene...
mRNA derived from bovine PBMC was analyzed to confirm the transcription of the multiple genes annotated above. To verify that cDNA sequences (designated with a prefix of 'CH') obtained were indeed representative of multiple genes, instead of genetic variation among animals, all reactions were performed using mRNA from a single animal. Also, because...
When we compared individual WC1 SRCR domains using cDNA and annotated genomic sequences by aligning deduced amino acid sequences we found the greatest variability among Domain 1's, with percent identities as low as 50.4% (Table 4). There was less variability among the other domains with Domain 9's sharing the most identity ranging from 88.2% to 99....
It could be reasoned that the most divergent of the 11 WC1 SRCR domains, i.e. Domain 1 as shown above, would be the ligand binding region of the WC1 co-receptor and thus that the WC1 Domain 1 sequence associated with a particular γδT cell could influence its response to various stimuli. However, it is also possible that variation in the intracytopl...
As mentioned above, RT-PCR products for WC1 transcripts yielded bands of approximately 2.7 kb and 2.2 kb in addition to the larger bands that most commonly contained full-length sequence. When the smaller bands were sequenced they were found to represent alternative splice variants. All 35 WC1 cDNA sequences obtained in this study are shown schemat...
- Carolyn T A Herzig, Cynthia L Baldwin
- 2009
Jun 30, 2023 · Both WCC subunits, WC1 and WC2, coprecipitated with 2xV5-HDA3 in samples from dark-grown and LP-exposed samples (Fig. 5E and fig. S4A). In contrast, neither WC1 nor WC2 coprecipitated with V5 antibody from extract prepared from a WT control strain, expressing untagged HDA3. The data indicate that HDA3 is a constitutive interactor of WCC.
Oct 28, 2009 · In this study, we found that differences existed in the 4 WC measurement sites (for boys, WC1 and WC4 < WC2; for girls, WC1, WC3, and WC4 < WC2) and that all sites were not significantly or equivalently associated with metabolic risk.
Jan 1, 1991 · The WC1 + cells are found in the blood, skin and spleen while the WC1 − γδ T cells predominate in the gut, mammary gland and uterus. In ruminants, γ δ T cells may produce IFNγ, IL-17, IL-10 and TGFβ, have cytotoxic activity and memory responses.
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What cDNA clone is found in wc1-11?
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Dec 25, 2001 · Here, we present a biochemical analysis of WC1 and WC2 polypeptides in N. crassa. Using specific antisera against WC1 and WC2, respectively, the subcellular localization of the WC polypeptides was investigated. The WC1 protein was localized exclusively in the nucleus, whereas WC2 was detected in both the nuclear and cytoplasmic fractions.
