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Standard applications : Masson's trichrome staining is widely used to study muscular pathologies ( muscular dystrophy ), cardiac pathologies ( infarct ), hepatic pathologies ( cirrhosis) or kidney pathologies (glomerular fibrosis). It can also be used to detect and analyze tumors on hepatic and kidney biopsies. [1] Variants.
Specifically, Masson's trichrome utilizes three stains to selectively identify muscle, collagen fibers, and fibrin. As illustrated in Figure 78.3, the fibrotic area is stained blue, indicating the area at risk. (Note: red = intact myocardium.)
COLLAGEN - MASSON'S TRICHROME STAIN(TRI) PURPOSE: Used to differentiate between collagen and smooth muscle in tumors, and the increase of collagen in diseases such as cirrhosis. Routine stain for liver and kidney biopsies. PRINCIPLE: As the name implies, three dyes are employed selectively staining muscle, collagen fibers, fibrin, and erythrocytes.
Jul 28, 2022 · Masson’s Trichrome Staining is a histological staining method used for selectively stain collagen, collagen fibers, fibrin, muscles, and erythrocytes. It uses three stains for staining hence the term Trichrome. These are Weigert’s Hematoxylin, Biebrich scarlet-acid fuschin solution, and Aniline blue.
Feb 26, 2018 · 9. 22K views 5 years ago Advice for Histotechnicians. Watch our new video on Masson's Trichrome Staining Protocol for Collagen Fibers. You will find the full staining procedure in detail, each ...
May 5, 2020 · The use of Masson’s trichrome staining of tissue sections from skeletal muscle is a fast method for detection of morphological alterations indicative of a fibrotic phenotype in this organ.
Jul 7, 2022 · Masson’s trichrome. Trichrome stains, as the name indicates, are mixture of three dyes used to differentiate the muscles, collagen fibers, fibrin and erythrocytes in connective tissue. One of the three dyes is usually nuclear stain and the other two dyes mainly differentiate collagen and muscle fibers.
